Document Detail

Involvement of aldolase A in X-ray resistance of human HeLa and UV(r)-1 cells.
MedLine Citation:
PMID:  18328256     Owner:  NLM     Status:  MEDLINE    
To find novel proteins involved in radio-resistance of human cells, we searched for nuclear proteins, whose expression levels alter after X-ray irradiation in HeLa cells, using agarose fluorescent two-dimensional differential gel electrophoresis following mass spectrometry. We identified 6 proteins, whose levels were increased in nuclei 24h after irradiation at 5Gy, including aldolase A. Nuclear aldolase A levels increased twofold after the irradiation, however, total aldolase A levels did not change. When the expression of aldolase A was suppressed by its specific siRNA, sensitization of the suppressed cells to X-ray-induced cell death was observed. In addition, UV(r)-1 cells with higher aldolase A expression exhibited lower sensitivity to X-ray-induced cell death than the parental RSa cells with lower aldolase A expression. These results suggest that aldolase A may play a role in the radio-response of human cells, probably in nuclei, in addition to its glycolytic role in the cytosol.
Jun Lu; Toshikazu Suzuki; Mamoru Satoh; Shiping Chen; Takeshi Tomonaga; Fumio Nomura; Nobuo Suzuki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-05
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  369     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-03-31     Completed Date:  2008-04-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  948-52     Citation Subset:  IM    
Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba 260-8670, Japan.
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MeSH Terms
Cell Nucleus / enzymology,  radiation effects
Fructose-Bisphosphate Aldolase / antagonists & inhibitors,  genetics,  physiology*
Hela Cells
RNA, Small Interfering / pharmacology
Radiation Tolerance* / drug effects,  genetics
Reg. No./Substance:
0/RNA, Small Interfering; EC Aldolase

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