Document Detail


Involvement of the acyl chain of ceramide in carbohydrate recognition by an anti-glycolipid monoclonal antibody: the case of an anti-melanoma antibody, M2590, to GM3-ganglioside.
MedLine Citation:
PMID:  2535499     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of the chain length of the fatty acid residue of the ceramide moiety of ganglioside GM3 on the binding ability of monoclonal antibody M2590, which is specific for the carbohydrate structure of GM3-ganglioside, was examined by means of a direct binding assay on thin layer chromatography plates (TLC immunostaining) and a quantitative enzyme-linked immunosorbent assay (ELISA). Derivatives of GM3 with a long fatty acid chain reacted with the M2590 antibody, but those with a short fatty acid chain showed no reaction in either assay system. These results suggested that the acyl fatty acid moiety of the ganglioside played an important role in the formation or maintenance of the antigenic structure of the carbohydrate moiety of the ganglioside.
Authors:
S Itonori; K Hidari; Y Sanai; M Taniguchi; Y Nagai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Glycoconjugate journal     Volume:  6     ISSN:  0282-0080     ISO Abbreviation:  Glycoconj. J.     Publication Date:  1989  
Date Detail:
Created Date:  1992-10-09     Completed Date:  1992-10-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8603310     Medline TA:  Glycoconj J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  551-60     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal*
Carbohydrate Sequence
Ceramides / analysis*,  immunology
Chromatography, Thin Layer
Dogs
Enzyme-Linked Immunosorbent Assay
Erythrocytes / chemistry
G(M3) Ganglioside / chemistry*,  immunology
Glycolipids / immunology*
Humans
Mass Spectrometry
Melanoma / immunology*
Molecular Sequence Data
Spectrometry, Mass, Fast Atom Bombardment
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Ceramides; 0/G(M3) Ganglioside; 0/Glycolipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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