Document Detail


Involvement of Trypomastigote Small Surface Antigen (TSSA) in Trypanosoma cruzi invasion of mammalian cells.
MedLine Citation:
PMID:  22428617     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Trypomastigote Small Surface Antigen (TSSA) is a polymorphic mucin-like molecule displayed on the surface of Trypanosoma cruzi trypomastigote forms. To evaluate its functional properties, we undertook comparative biochemical and genetic approaches on isoforms present in parasite stocks from extant evolutionary lineages (CL Brener and Sylvio X-10). We show that CL Brener TSSA, but not the Sylvio X-10 counterpart, exhibits dose-dependent and saturable binding toward non-macrophagic cell lines. This binding trigger Ca2+-based signaling responses in the target cell while providing an anchor for the invading parasite. Accordingly, exogenous addition of either TSSA-derived peptides or specific antibodies significantly inhibits invasion of CL Brener but not Sylvio X-10 trypomastigotes. Non-infective epimastigote forms -that do not express detectable levels of TSSA- were stably transfected with TSSA cDNA from either parasite stock. Although both transfectants produced a surface-associated, mucin-like TSSA product, epimastigotes expressing CL Brener TSSA showed a ~2-fold increase in their attachment to mammalian cells. Overall, these findings indicate that CL Brener TSSA functions as a parasite adhesin, engaging surface receptor(s) and inducing signaling pathways on the host cell as a pre-requisite for parasite internalization. More importantly, the contrasting functional features of TSSA isoforms provide one appealing mechanism underlying the differential infectivity of T. cruzi stocks.
Authors:
Gaspar E Cánepa; Maria Sol Degese; Alexandre Budu; Celia R Garcia; Carlos A Buscaglia
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-20
Journal Detail:
Title:  The Biochemical journal     Volume:  -     ISSN:  1470-8728     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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