Document Detail


Involvement of Rho-type GTPase in control of cell size in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  17302939     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Maintaining specific cell size, which is important for many organisms, is achieved by coordinating cell growth and cell division. In the budding yeast Saccharomyces cerevisiae, the existence of two cell-size checkpoints is proposed: at the first checkpoint, cell size is monitored before budding at the G1/S transition, and at the second checkpoint, actin depolymerization occurring in the small bud is monitored before the G2/M transition. Morphological analyses have revealed that the small GTPase Rho1p participates in cell-size control at both the G1/S and the G2/M boundaries. One group of rho1 mutants (rho1A) underwent premature entry into mitosis, leading to the birth of abnormally small cells. In another group of rho1 mutants (rho1B), the mother cells failed to reach an appropriate size before budding, and expression of the G1 cyclin Cln2p began at an earlier phase of the cell cycle. Analyses of mutants defective in Rho1p effector proteins indicate that Skn7p, Fks1p and Mpk1p are involved in cell-size control. Thus, Rho1p and its downstream regulatory pathways are involved in controlling cell size in S. cerevisiae.
Authors:
Yo Kikuchi; Eri Mizuuchi; Satoru Nogami; Shinichi Morishita; Yoshikazu Ohya
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-16
Journal Detail:
Title:  FEMS yeast research     Volume:  7     ISSN:  1567-1356     ISO Abbreviation:  FEMS Yeast Res.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-21     Completed Date:  2007-07-31     Revised Date:  2009-07-28    
Medline Journal Info:
Nlm Unique ID:  101085384     Medline TA:  FEMS Yeast Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  569-78     Citation Subset:  IM    
Affiliation:
Department of Integrated Biosciences, University of Tokyo, Kashiwa, Chiba, Japan.
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MeSH Terms
Descriptor/Qualifier:
Cyclins / metabolism
Image Processing, Computer-Assisted
MAP Kinase Signaling System
Mitosis
Mutation
Saccharomyces cerevisiae / cytology,  genetics,  physiology*
Saccharomyces cerevisiae Proteins / genetics,  physiology*
rho GTP-Binding Proteins / genetics,  physiology*
Chemical
Reg. No./Substance:
0/CLN2 protein, S cerevisiae; 0/Cyclins; 0/Saccharomyces cerevisiae Proteins; EC 3.6.5.2/RHO1 protein, S cerevisiae; EC 3.6.5.2/rho GTP-Binding Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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