| Involvement of Rho-kinase in collar-induced vasoconstriction and vascular hypersensitivity to serotonin in rat carotid. | |
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MedLine Citation:
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PMID: 19942308 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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BACKGROUND: Vasoconstriction and vascular hypersensitivity to serotonin has been described previously in animal models of adventitia injury. The present study was undertaken to investigate the contribution of the RhoA/Rho-kinase pathway to the collar-induced the change of vascular contractility and reactivity in rat carotid artery. METHODS: Wistar Kyoto rats were assigned to 4 treatments (n=12): vehicle, fasudil, valsartan, and fasudil plus valsartan. After 1week of treatment, adventitia injury was induced by positioning a silicone collar around the right carotid artery for 1week. Blood flow and vascular reactivity to serotonin was determined 1week after injury, and carotids were harvested for morphometry and biochemical analysis. RESULTS: Adventitia injury leaded to histological changes of vasoconstriction with the percent lumen patency of 54.5±4.3% (p<0.001) decreasing, accompanying by the reduction of the blood flow (2.79±0.22ml/min vs. 3.67±0.26 mi/min，p<0.001) when compared to contralateral arteries. The increase of vascular reactivity sensitivity to serotonin was observed in the collared artery when compared with the contralateral artery. Treatment with valsartan and fasudil prevented the development of vasoconstriction, improved the carotid blood flow and normalized the hypersensitivity to serotonin. Injury increased Angiotensin II type 1（AT(1)）receptor, Rho-kinase, and p-MYPT1(Thr696) expression. Valsartan lowered the Rho-kinase and p-MYPT1(Thr696) expression. Fasudil inhibited the p-MYPT1(Thr696) expression. CONCLUSIONS: Collar-induced adventitia injury resulted in the enhancement of vascular contractility and reactivity. The activation of RhoA/Rho-kinase signal pathway, stimulated by AT(1) receptor, plays an important role in the collar-induced the change of vascular contractility and reactivity. |
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Authors:
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Lianna Xie; Dingyin Zeng; Haishan Zhang; Danmeng Sun; Xuefeng Pang; Qigang Guan |
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Publication Detail:
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Type: Journal Article Date: 2009-11-26 |
Journal Detail:
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Title: International journal of cardiology Volume: 148 ISSN: 1874-1754 ISO Abbreviation: Int. J. Cardiol. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8200291 Medline TA: Int J Cardiol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 168-73 Citation Subset: IM |
Copyright Information:
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Copyright © 2009 Elsevier Ireland Ltd. All rights reserved. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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