Document Detail


Involvement of Ras and AP-1 in Helicobacter pylori-induced expression of COX-2 and iNOS in gastric epithelial AGS cells.
MedLine Citation:
PMID:  19495976     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Helicobacter pylori (H. pylori) is an important risk factor for chronic gastritis, peptic ulcer, and gastric cancer. The genetic differences of H. pylori isolates play a role in the clinical outcome of the infection. Inflammatory genes including cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) are involved in H. pylori gastritis. Transcription factor AP-1 is composed of c-Fos and c-Jun and mediates inflammation and carcinogenesis. Ras acts as a regulator for AP-1 activation in various cells. We investigated whether H. pylori in a Korean isolate (HP99), a cagA ( + ), vacA ( + ) strain, induces the expression of c-Fos and c-Jun for AP-1 activation to induce COX-2 and iNOS and whether HP99-induced expressions of COX-2 and iNOS are mediated by Ras and AP-1, determined by the expressions of c-Fos and c-Jun, in gastric epithelial AGS cells, using transfection with mutant genes for Ras (ras N-17) and c-Jun (TAM-67). As a result, HP99 induced the expression of c-Fos and c-Jun and the expressions of COX-2 and iNOS in AGS cells. Transfection with mutant genes for Ras or c-Jun suppressed HP99-induced expressions of COX-2 and iNOS in AGS cells. In conclusion, H. pylori in a Korean isolate induces the expression of COX-2 and iNOS via AP-1 activation, which may be mediated by Ras and the expression of c-Fos and c-Jun in gastric epithelial cells.
Authors:
Soon Ok Cho; Joo Weon Lim; Kyung Hwan Kim; Hyeyoung Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-03
Journal Detail:
Title:  Digestive diseases and sciences     Volume:  55     ISSN:  1573-2568     ISO Abbreviation:  Dig. Dis. Sci.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-18     Completed Date:  2010-04-02     Revised Date:  2011-10-27    
Medline Journal Info:
Nlm Unique ID:  7902782     Medline TA:  Dig Dis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  988-96     Citation Subset:  AIM; IM    
Affiliation:
Department of Pharmacology, Brain Korea 21 Project for Medical Sciences, Seoul, 120-752, Korea.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / genetics*,  pathology,  virology
Cell Line, Tumor
Cyclooxygenase 2 / genetics*
Dinoprostone / metabolism
Enzyme Induction / genetics
Epithelial Cells / metabolism,  virology
Gastric Mucosa / metabolism,  virology
Gastritis / genetics*,  pathology,  virology
Gene Expression Regulation / genetics
Helicobacter Infections / genetics*,  virology
Helicobacter pylori / genetics*
Humans
Nitric Oxide Synthase Type II / genetics*
Nitrites / metabolism
Peptic Ulcer / genetics*,  pathology*,  virology
Proto-Oncogene Proteins c-fos / genetics*
Proto-Oncogene Proteins c-jun / genetics*
RNA, Messenger / genetics
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction / genetics
Stomach Neoplasms / genetics*,  pathology,  virology
Transcription Factor AP-1 / physiology*
Transfection
ras Proteins / physiology*
Chemical
Reg. No./Substance:
0/Nitrites; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 0/RNA, Messenger; 0/Transcription Factor AP-1; 363-24-6/Dinoprostone; EC 1.14.13.39/NOS2 protein, human; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/PTGS2 protein, human; EC 3.6.5.2/ras Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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