Document Detail

Involvement of PKC-iota in glioma proliferation.
MedLine Citation:
PMID:  18211289     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: The objectives of our research were to determine whether expression of PKC-iota is altered either in gliomas or in benign and malignant meningiomas, compared to normal brain. In addition, we wished to establish the expression of PKC-iota in proliferating plus in cell cycle-arrested glioma cell lines, as well as the relationship between PKC-iota siRNA on PKC-iota protein content and cell proliferation.
MATERIALS AND METHODS: Western blot analyses for PKC-iota were performed on 12 normal brain biopsies, 15 benign meningiomas, three malignant meningiomas and three gliomas.
RESULTS: Results demonstrated no (n = 9) or very weak (n = 3) detection of PKC-iota in normal brain tissue. In comparison, PKC-iota was robustly present in the majority of the benign meningiomas. Similarly, PKC-iota was abundant in all malignant meningiomas and gliomas. Western blotting for PKC-iota in confluent or proliferating glioma cell lines depicted substantial quantities of PKC-iota in proliferating T98G and U-138MG glioma cells. In contrast, confluent cells had either 71% (T98G) or 21% (U-138MG) less PKC-iota than proliferating cells. T98 and U-138 MG glioma cells treated with 100 nm PKC-iota siRNA had lower levels of cell proliferation compared to control siRNA-A and complete down-regulation of PKC-iota protein content.
CONCLUSION: These results support the concept that presence of PKC-iota may be required for cell proliferation to take place.
R Patel; H Win; S Desai; K Patel; J A Matthews; M Acevedo-Duncan
Related Documents :
20702249 - Studies of human bronchial epithelium in vitro: changes of [ca(2+)](i) in relation to i...
10484519 - Cyclooxygenase-2 expression and function in the medullary thick ascending limb.
12187329 - Effect of novel modulators of protein kinase c activity upon chemotherapy-induced diffe...
2920299 - Effects of tetradecanoyl phorbol acetate, pyrethroids and ddt in the v79.
287029 - Tumor promoter-mediated inhibition of cell differentiation: suppression of the expressi...
1525349 - Protein kinase c activation in murine endothelioma cell lines containing middle t antig...
24558429 - Mir-184 post-transcriptionally regulates sox7 expression and promotes cell proliferatio...
21372419 - Invasion inhibitors of human fibrosarcoma ht 1080 cells from the rhizomes of zingiber c...
2482279 - Galanin and vasoactive intestinal polypeptide are colocalised with classical pituitary ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Cell proliferation     Volume:  41     ISSN:  1365-2184     ISO Abbreviation:  Cell Prolif.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-23     Completed Date:  2008-03-31     Revised Date:  2012-06-13    
Medline Journal Info:
Nlm Unique ID:  9105195     Medline TA:  Cell Prolif     Country:  England    
Other Details:
Languages:  eng     Pagination:  122-35     Citation Subset:  IM    
Department of Chemistry, University of South Florida, and James A. Haley Veterans Hospital, Tampa, FL 33612, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Base Sequence
Brain Neoplasms / enzymology,  pathology*
Cell Line, Tumor
Cell Proliferation
Flow Cytometry
Glioma / enzymology,  pathology*
Isoenzymes / metabolism*
Protein Kinase C / metabolism*
RNA, Small Interfering
Reg. No./Substance:
0/Isoenzymes; 0/RNA, Small Interfering; EC Kinase C; EC kinase C lambda

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Inhibition of transient receptor potential canonical channels impairs cytokinesis in human malignant...
Next Document:  Identification of genes deregulated during serum-free medium adaptation of a Burkitt's lymphoma cell...