Document Detail


Involvement of PKC-iota in glioma proliferation.
MedLine Citation:
PMID:  18211289     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: The objectives of our research were to determine whether expression of PKC-iota is altered either in gliomas or in benign and malignant meningiomas, compared to normal brain. In addition, we wished to establish the expression of PKC-iota in proliferating plus in cell cycle-arrested glioma cell lines, as well as the relationship between PKC-iota siRNA on PKC-iota protein content and cell proliferation.
MATERIALS AND METHODS: Western blot analyses for PKC-iota were performed on 12 normal brain biopsies, 15 benign meningiomas, three malignant meningiomas and three gliomas.
RESULTS: Results demonstrated no (n = 9) or very weak (n = 3) detection of PKC-iota in normal brain tissue. In comparison, PKC-iota was robustly present in the majority of the benign meningiomas. Similarly, PKC-iota was abundant in all malignant meningiomas and gliomas. Western blotting for PKC-iota in confluent or proliferating glioma cell lines depicted substantial quantities of PKC-iota in proliferating T98G and U-138MG glioma cells. In contrast, confluent cells had either 71% (T98G) or 21% (U-138MG) less PKC-iota than proliferating cells. T98 and U-138 MG glioma cells treated with 100 nm PKC-iota siRNA had lower levels of cell proliferation compared to control siRNA-A and complete down-regulation of PKC-iota protein content.
CONCLUSION: These results support the concept that presence of PKC-iota may be required for cell proliferation to take place.
Authors:
R Patel; H Win; S Desai; K Patel; J A Matthews; M Acevedo-Duncan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Cell proliferation     Volume:  41     ISSN:  1365-2184     ISO Abbreviation:  Cell Prolif.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-23     Completed Date:  2008-03-31     Revised Date:  2012-06-13    
Medline Journal Info:
Nlm Unique ID:  9105195     Medline TA:  Cell Prolif     Country:  England    
Other Details:
Languages:  eng     Pagination:  122-35     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of South Florida, and James A. Haley Veterans Hospital, Tampa, FL 33612, USA.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Brain Neoplasms / enzymology,  pathology*
Cell Line, Tumor
Cell Proliferation
Flow Cytometry
Glioma / enzymology,  pathology*
Humans
Isoenzymes / metabolism*
Protein Kinase C / metabolism*
RNA, Small Interfering
Chemical
Reg. No./Substance:
0/Isoenzymes; 0/RNA, Small Interfering; EC 2.7.11.13/Protein Kinase C; EC 2.7.11.13/protein kinase C lambda

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