Document Detail

Involvement of the N-terminus of Bax in its intracellular localization and function.
MedLine Citation:
PMID:  11852059     Owner:  NLM     Status:  MEDLINE    
We have identified, using site-directed mutagenesis, a proline located at position 13 of Baxalpha (Bax) as crucial for the maintenance of its cytosolic conformation. The substitution of this proline by a valine results in a strong binding of Bax to mitochondria and to conformational changes monitored by a decreased sensitivity of Bax to mild proteolysis and the enhancement of its oligomerization state. Deletion of the C-terminus of Bax does not modify its intracellular localization. On the other hand, the pro-apoptotic activity of Bax is enhanced by a deletion of the C-terminus in the absence of the N-terminus but is decreased in its presence. These results suggest that both extremities functionally interact to control the activity but not the subcellular localization of Bax.
P-F Cartron; C Moreau; L Oliver; E Mayat; K Meflah; F M Vallette
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  512     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-02-19     Completed Date:  2002-03-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  95-100     Citation Subset:  IM    
INSERM U419, IFR 26, 9 quai MONCOUSU, 44035 Cedex, Nantes, France.
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MeSH Terms
Amino Acid Sequence
BH3 Interacting Domain Death Agonist Protein
Carrier Proteins / metabolism
Cell Compartmentation*
Cytosol / metabolism
Intracellular Membranes / metabolism
Mitochondria, Liver / metabolism
Molecular Sequence Data
Proline / genetics,  metabolism
Protein Binding
Protein Structure, Tertiary
Protein Transport
Proto-Oncogene Proteins / genetics,  metabolism*
Proto-Oncogene Proteins c-bcl-2*
bcl-2-Associated X Protein
Reg. No./Substance:
0/BH3 Interacting Domain Death Agonist Protein; 0/Carrier Proteins; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 147-85-3/Proline

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