| Involvement of mitochondrial ferredoxin and para-aminobenzoic acid in yeast coenzyme Q biosynthesis. | |
| | |
MedLine Citation:
|
PMID: 20534343 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Yeast ubiquinone or coenzyme Q(6) (Q(6)) is a redox active lipid that plays a crucial role in the mitochondrial electron transport chain. At least nine proteins (Coq1p-9p) participate in Q(6) biosynthesis from 4-hydroxybenzoate (4-HB). We now show that the mitochondrial ferredoxin Yah1p and the ferredoxin reductase Arh1p are required for Q(6) biosynthesis, probably for the first hydroxylation of the pathway. Conditional Gal-YAH1 and Gal-ARH1 mutants accumulate 3-hexaprenyl-4-hydroxyphenol and 3-hexaprenyl-4-aminophenol. Para-aminobenzoic acid (pABA) is shown to be the precursor of 3-hexaprenyl-4-aminophenol and to compete with 4-HB for the prenylation reaction catalyzed by Coq2p. Yeast cells convert U-((13)C)-pABA into (13)C ring-labeled Q(6), a result that identifies pABA as a new precursor of Q(6) and implies an additional NH(2)-to-OH conversion in Q(6) biosynthesis. Our study identifies pABA, Yah1p, and Arh1p as three actors in Q(6) biosynthesis. |
| | |
Authors:
|
Fabien Pierrel; Olivier Hamelin; Thierry Douki; Sylvie Kieffer-Jaquinod; Ulrich Mühlenhoff; Mohammad Ozeir; Roland Lill; Marc Fontecave |
Related Documents
:
|
2849603 - Effects of fleroxacin on hela cell functions and topoisomerase ii. 553453 - Convulsions from excessive dosage of nalidixic acid: a case report. 8097773 - Investigation of synergism between combinations of ciprofloxacin, polymyxin, sulphadiaz... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Chemistry & biology Volume: 17 ISSN: 1879-1301 ISO Abbreviation: Chem. Biol. Publication Date: 2010 May |
Date Detail:
|
Created Date: 2010-06-10 Completed Date: 2010-09-15 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9500160 Medline TA: Chem Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 449-59 Citation Subset: IM |
Copyright Information:
|
2010 Elsevier Ltd. All rights reserved. |
Affiliation:
|
Laboratoire de Chimie et Biologie des Métaux, UMR5249 CNRS-CEA-UJF, CEA Grenoble, F-38054 Grenoble Cedex 9, France. fabien.pierrel@cea.fr |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
4-Aminobenzoic Acid
/
metabolism* Adrenodoxin / metabolism* Aminophenols / metabolism Ferredoxin-NADP Reductase / metabolism* Membrane Proteins / metabolism* Mitochondria / metabolism* Mutation Oxidation-Reduction Parabens / metabolism* Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / metabolism* Ubiquinone / biosynthesis* |
| Chemical | |
Reg. No./Substance:
|
0/Aminophenols; 0/Membrane Proteins; 0/Parabens; 0/Saccharomyces cerevisiae Proteins; 0/YAH1 protein, S cerevisiae; 123-30-8/4-aminophenol; 12687-22-8/Adrenodoxin; 1339-63-5/Ubiquinone; 150-13-0/4-Aminobenzoic Acid; 99-96-7/4-hydroxybenzoic acid; EC 1.18.1.2/Arh1 protein, S cerevisiae; EC 1.18.1.2/Ferredoxin-NADP Reductase |
| Comments/Corrections | |
Comment In:
|
Chem Biol. 2010 May 28;17(5):415-6
[PMID:
20534338
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Energy conversion in natural and artificial photosynthesis.
Next Document: Application of Proteomic Profiling Based on 2D-DIGE for Classification of Compounds According to the...