Document Detail


Involvement of mitochondrial ferredoxin and para-aminobenzoic acid in yeast coenzyme Q biosynthesis.
MedLine Citation:
PMID:  20534343     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Yeast ubiquinone or coenzyme Q(6) (Q(6)) is a redox active lipid that plays a crucial role in the mitochondrial electron transport chain. At least nine proteins (Coq1p-9p) participate in Q(6) biosynthesis from 4-hydroxybenzoate (4-HB). We now show that the mitochondrial ferredoxin Yah1p and the ferredoxin reductase Arh1p are required for Q(6) biosynthesis, probably for the first hydroxylation of the pathway. Conditional Gal-YAH1 and Gal-ARH1 mutants accumulate 3-hexaprenyl-4-hydroxyphenol and 3-hexaprenyl-4-aminophenol. Para-aminobenzoic acid (pABA) is shown to be the precursor of 3-hexaprenyl-4-aminophenol and to compete with 4-HB for the prenylation reaction catalyzed by Coq2p. Yeast cells convert U-((13)C)-pABA into (13)C ring-labeled Q(6), a result that identifies pABA as a new precursor of Q(6) and implies an additional NH(2)-to-OH conversion in Q(6) biosynthesis. Our study identifies pABA, Yah1p, and Arh1p as three actors in Q(6) biosynthesis.
Authors:
Fabien Pierrel; Olivier Hamelin; Thierry Douki; Sylvie Kieffer-Jaquinod; Ulrich Mühlenhoff; Mohammad Ozeir; Roland Lill; Marc Fontecave
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chemistry & biology     Volume:  17     ISSN:  1879-1301     ISO Abbreviation:  Chem. Biol.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-06-10     Completed Date:  2010-09-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9500160     Medline TA:  Chem Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  449-59     Citation Subset:  IM    
Copyright Information:
2010 Elsevier Ltd. All rights reserved.
Affiliation:
Laboratoire de Chimie et Biologie des Métaux, UMR5249 CNRS-CEA-UJF, CEA Grenoble, F-38054 Grenoble Cedex 9, France. fabien.pierrel@cea.fr
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MeSH Terms
Descriptor/Qualifier:
4-Aminobenzoic Acid / metabolism*
Adrenodoxin / metabolism*
Aminophenols / metabolism
Ferredoxin-NADP Reductase / metabolism*
Membrane Proteins / metabolism*
Mitochondria / metabolism*
Mutation
Oxidation-Reduction
Parabens / metabolism*
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae Proteins / metabolism*
Ubiquinone / biosynthesis*
Chemical
Reg. No./Substance:
0/Aminophenols; 0/Membrane Proteins; 0/Parabens; 0/Saccharomyces cerevisiae Proteins; 0/YAH1 protein, S cerevisiae; 123-30-8/4-aminophenol; 12687-22-8/Adrenodoxin; 1339-63-5/Ubiquinone; 150-13-0/4-Aminobenzoic Acid; 99-96-7/4-hydroxybenzoic acid; EC 1.18.1.2/Arh1 protein, S cerevisiae; EC 1.18.1.2/Ferredoxin-NADP Reductase
Comments/Corrections
Comment In:
Chem Biol. 2010 May 28;17(5):415-6   [PMID:  20534338 ]

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