| Involvement of the JAK-STAT pathway and SOCS3 in the regulation of adiponectin-generated reactive oxygen species in murine macrophage RAW 264 cells. | |
| | |
MedLine Citation:
|
PMID: 20564237 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Adiponectin is a protein hormone produced by differentiating adipocytes and has been proposed to have anti-diabetic and immunosuppressive properties. We previously reported that the globular form of adiponectin (gAd) induces the generation of reactive oxygen species (ROS) and nitric oxide (NO), followed by caspase-dependent apoptotic cell death in RAW 264 cells. Here, we demonstrate that gAd-induced ROS generation and apoptosis were diminished by suppressor of cytokine signaling 3 (SOCS3). The phosphorylation level of signal transducer and activator of transcription (STAT) 3 detected by Western blotting was highest at 20 min in gAd-treated RAW 264 cells. This phosphorylation was inhibited by AG490, a specific inhibitor of janus-activator kinase (JAK). The gAd-induced ROS and NO were reduced by administration of AG490 and Jak-2-specific siRNA in RAW 264 cells. The gAd stimulation transiently induced SOCS3 mRNA expression and protein production. We examined SOCS3-overexpressing RAW 264 cells to investigate the role of the JAK-STAT pathway in gAd-induced ROS and NO generation. SOCS3 overexpression significantly reduced both ROS and NO generation. Additionally, gAd-induced caspase activation and apoptotic cell death were reduced in SOCS3 transfectants compared with vector control transfectants. These results suggest that the JAK-STAT pathway, which can be suppressed by SOCS3 expression, is involved in gAd-induced ROS and NO generation followed by apoptotic cell death. |
| | |
Authors:
|
Sumio Akifusa; Noriaki Kamio; Yoshihiro Shimazaki; Noboru Yamaguchi; Kazuaki Nonaka; Yoshihisa Yamashita |
Related Documents
:
|
15916847 - Reactive oxygen species: biological stimuli of neuroblastoma cell response. 19376147 - Chromium picolinate induced apoptosis of lymphocytes and the signaling mechanisms thereof. 12388087 - Mitochondrial oxidant production by a pollutant dust and no-mediated apoptosis in human... 17624697 - The diabetogenic effects of the combination of humic acid and arsenic: in vitro and in ... 21711157 - Favorable response of chronic refractory immune thrombocytopenic purpura to mesenchymal... 20488537 - Cultivating hepatocytes on printed arrays of hgf and bmp7 to characterize protective ef... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of cellular biochemistry Volume: 111 ISSN: 1097-4644 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2010 Oct |
Date Detail:
|
Created Date: 2010-09-27 Completed Date: 2011-01-31 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
|
Languages: eng Pagination: 597-606 Citation Subset: IM |
Copyright Information:
|
© 2010 Wiley-Liss, Inc. |
Affiliation:
|
Faculty of Dental Science, Kyushu University, Fukuoka, Japan. akifusa@dent.kyushu-u.ac.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adiponectin
/
metabolism* Animals Apoptosis Cell Line Janus Kinases / metabolism* Macrophages / metabolism* Mice Nitric Oxide / metabolism Phosphorylation RNA, Messenger / analysis Reactive Oxygen Species / metabolism* STAT Transcription Factors / metabolism Signal Transduction Suppressor of Cytokine Signaling Proteins / biosynthesis, genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Adiponectin; 0/RNA, Messenger; 0/Reactive Oxygen Species; 0/STAT Transcription Factors; 0/Socs3 protein, mouse; 0/Suppressor of Cytokine Signaling Proteins; 10102-43-9/Nitric Oxide; EC 2.7.10.2/Janus Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Differentiation of cardiomyocytes from human embryonic stem cells is accompanied by changes in the e...
Next Document: Abnormal microarchitecture and reduced stiffness at the radius and tibia in postmenopausal women wit...