Document Detail

Involvement of glial cells in the autoregulation of optic nerve head blood flow in rabbits.
MedLine Citation:
PMID:  22589427     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To investigate the involvement of glial cells in the autoregulation of optic nerve head (ONH) blood flow in response to elevated intraocular pressure (IOP).
METHODS: Rabbit eyes were treated with an intravitreal injection of l-2-aminoadipic acid (LAA), a gliotoxic compound. Twenty-four hours after the injection IOP was artificially elevated from a baseline of 20 to 50 or 70 mm Hg and maintained at each IOP level for 30 minutes. ONH blood flow was measured by laser speckle flowgraphy every 10 minutes. Ocular perfusion pressure (OPP) was calculated to investigate the relationship between ONH blood flow and OPP. To evaluate the effects of LAA on the function and morphology of retinal neurons and glial cells, electroretinogram (ERG) was monitored after injections of LAA (2.0 and 6.0 mM) or saline as a control. Histologic and immunohistochemical examinations were then performed.
RESULTS: In the LAA-treated eyes, histologic changes selectively occurred in the retinal Müller cells and ONH astrocytes. There was not any significant reduction of amplitude or elongation of implicit time of each parameter in the ERG after LAA injection compared with control. ONH blood flow in LAA-treated eyes was significantly decreased with a reduction of OPP during IOP elevation to 50 and 70 mm Hg, whereas blood flow was maintained in control eyes during IOP elevation to 50 mm Hg.
CONCLUSIONS: These results indicate the involvement of glial ells in the autoregulation of ONH blood flow during IOP elevation.
Maho Shibata; Tetsuya Sugiyama; Takuji Kurimoto; Hidehiro Oku; Takashi Okuno; Takatoshi Kobayashi; Tsunehiko Ikeda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-06-20
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  53     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-06-22     Completed Date:  2012-09-10     Revised Date:  2013-01-09    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3726-32     Citation Subset:  IM    
Department of Ophthalmology, Osaka Medical College, Takatsuki, Osaka, Japan.
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MeSH Terms
2-Aminoadipic Acid / pharmacology
Case-Control Studies
Excitatory Amino Acid Antagonists / pharmacology
Neuroglia / drug effects,  physiology*
Ocular Hypertension / physiopathology*
Optic Disk / blood supply*
Regional Blood Flow / physiology
Reg. No./Substance:
0/Excitatory Amino Acid Antagonists; 542-32-5/2-Aminoadipic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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