Document Detail


Involvement of ETS1 in thioredoxin-binding protein 2 transcription induced by a synthetic retinoid CD437 in human osteosarcoma cells.
MedLine Citation:
PMID:  19932085     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CD437, a synthetic retinoid, has a potent antitumor activity, in which an RAR-independent mechanism may be involved. Our previous study showed that CD437 transcriptionally upregulates the expression of thioredoxin-binding protein 2 (TBP2), leading to c-Jun N-terminal kinase 1 (JNK1)-mediated apoptosis. In the present study, we addressed the mechanism, by which CD437 induces TBP2 mRNA expression. CD437 efficiently caused the cell death of human osteosarcoma cells via apoptosis. CD437 also induced JNK1 activation through the upregulation of TBP2 mRNA, in consistent with our previous observation. A luciferase reporter assay for TBP2 promoter activation suggested that CD437-regulated TBP2 mRNA transcription requires the region between -400 and -300, which contains multiple possible ETS-binding sites. Finally, we demonstrated CD437-dependent recruitment of ETS1 transcription factor to this region by chromatin immunoprecipitation assay. These data suggest that ETS1 is involved in CD437-induced TBP2 mRNA expression in human osteosarcoma MG-63 cells.
Authors:
Koichi Hashiguchi; Hiroyuki Tsuchiya; Akiko Tomita; Chisa Ueda; Yuji Akechi; Tomohiko Sakabe; Akihiro Kurimasa; Masami Nozaki; Toshiyuki Yamada; Shigeki Tsuchida; Goshi Shiota
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Publication Detail:
Type:  Journal Article     Date:  2009-11-20
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  391     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-01-27     Completed Date:  2010-03-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  621-6     Citation Subset:  IM    
Copyright Information:
Copyright 2009. Published by Elsevier Inc.
Affiliation:
Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, Nishi-cho 86, Yonago 683-8504, Japan.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / pharmacology*
Apoptosis*
Carrier Proteins / genetics*
Cell Line, Tumor
Chromatin Immunoprecipitation
Humans
Neoplasms / metabolism*
Promoter Regions, Genetic
Proto-Oncogene Protein c-ets-1 / metabolism*
Retinoids / pharmacology*
Transcription, Genetic / drug effects*
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/CD 437; 0/Carrier Proteins; 0/ETS1 protein, human; 0/Proto-Oncogene Protein c-ets-1; 0/Retinoids; 0/TXNIP protein, human

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