| Involvement of E-cadherin in early in vitro development of adult and juvenile sheep embryos. | |
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MedLine Citation:
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PMID: 20047732 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The oocyte-to-embryo transition in mammals depends on maternal proteins and transcripts, which accumulate during oocyte differentiation. The aim of the present study was to examine the role of the junctional proteins beta-catenin and E-cadherin during preimplantation in vitro embryo development in sheep, comparing the competence of adult and prepubertal oocytes. We analysed the concentration of beta-catenin and E-cadherin in immature and in vitro-matured oocytes. There was a significant increase in E-cadherin concentration after 24 h of in vitro maturation and this was lower in prepubertal oocytes than in adult ones. We therefore studied the expression and distribution of E-cadherin during the major transition from maternal to embryonic genome. E-cadherin distribution and localisation in sheep was age- and developmental-stage dependent and was related to developmental kinetics. In fact, in adults, the majority of embryos showed the proper distribution of E-cadherin just beneath the membrane surfaces of all blastomeres and the percentage of embryos with this distribution increased with the increase in cell number during development. On the contrary, and regardless of their developmental stage, the majority of prepubertal embryos showed an uneven distribution of the protein, often associated with the occurrence of cellular fragmentation. In conclusion, our results suggest that E-cadherin plays a pivotal role during preimplantation embryo growth in sheep and may be one of the possible cytoplasmic factors involved in the reduced developmental competence of prepubertal female gametes. |
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Authors:
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Silvia Modina; Giovanni G Leoni; Valentina Lodde; Salvatore Naitana; Silvia Pirani; Sara Succu; Fiammetta Berlinguer; Alberto Maria Luciano |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Reproduction, fertility, and development Volume: 22 ISSN: 1031-3613 ISO Abbreviation: Reprod. Fertil. Dev. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-01-05 Completed Date: 2010-04-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8907465 Medline TA: Reprod Fertil Dev Country: Australia |
Other Details:
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Languages: eng Pagination: 468-77 Citation Subset: IM |
Affiliation:
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Department of Animal Sciences, University of Milan, Milan 20122, Italy. silvia.modina@unimi.it |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cadherins / physiology* Embryonic Development / physiology* Female Fertilization in Vitro Immunohistochemistry Male Sexual Maturation / physiology Sheep / embryology*, physiology* beta Catenin / physiology |
| Chemical | |
Reg. No./Substance:
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0/Cadherins; 0/beta Catenin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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