| Involvement of calmodulin kinase II in the action of sulphur mustard on the contraction of vascular smooth muscle. | |
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MedLine Citation:
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PMID: 20735375 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Sulphur mustard (SM) is an alkylating agent whose mechanism is not fully understood. To investigate the early action of SM, we examined the effect of SM on contraction of vascular smooth muscles. Phenylephrine (PE)-induced contraction was reduced by SM, but only marginally by 70 mM KCl(-) . Additional reduction was induced by nifedipine in SM-treated arteries. In the absence of extracellular Ca(2+) , contraction of arteries by PE was reduced, which was fully recovered by addition of 2 mM Ca(2+) . However, recovery was attenuated by pre-treatment with SM. The effect of SM on contraction by PE was not influenced by pre- and post-treatment with Phorbol 12, 13-dibutyrate. Calmodulin kinase II (CaMKII) was implicated as being responsible for the action of SM, because the contractile mechanisms of vascular smooth muscle via both Ca(2+) -calmodulin-myosin light chain kinase axis and protein kinase C-proline-rich tyrosine kinase axis were not related to the action of SM. Elevation of phosphorylated CaMKII level by Ionomycin or PE was attenuated by treatment of SM on western blot. CaMKII may be a candidate target molecule of SM in early stage contraction of vascular smooth muscle. |
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Authors:
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Min Tae Kim; Woo-Jae Park; Shin Kim; Ji Won Lee; Sang Yun Lee; Ju-Hong Jeon; Insuk So; Byung Joo Kim; Seon Jeong Kim |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Basic & clinical pharmacology & toxicology Volume: 108 ISSN: 1742-7843 ISO Abbreviation: Basic Clin. Pharmacol. Toxicol. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-16 Completed Date: 2011-01-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101208422 Medline TA: Basic Clin Pharmacol Toxicol Country: Denmark |
Other Details:
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Languages: eng Pagination: 28-33 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Basic & Clinical Pharmacology & Toxicology © 2010 Nordic Pharmacological Society. |
Affiliation:
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Department of Rehabilitation Medicine, Kwandong University of Medicine, Goyang, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alkylating Agents
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toxicity* Animals Calcium-Calmodulin-Dependent Protein Kinase Type 2 / metabolism* Chemical Warfare Agents / toxicity Female Male Muscle Contraction / drug effects* Muscle, Smooth, Vascular / drug effects*, enzymology Mustard Gas / toxicity* Rabbits Rats Rats, Sprague-Dawley Signal Transduction / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Alkylating Agents; 0/Chemical Warfare Agents; 505-60-2/Mustard Gas; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinase Type 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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