| Involvement of the Bcl-2 family members in Pinus massoniana bark extract induced apoptosis in HeLa cells. | |
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MedLine Citation:
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PMID: 18803251 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pinus massoniana bark extract (PMBE) contains a variety of flavonoids whose antioxidant properties have been confirmed in vitro. This study was undertaken to evaluate the cytotoxic effects and the mechanism of cell death on the PMBE-treated human cervical cancer cell line, HeLa. PMBE treatment led to cell growth inhibition in a dose- and time-dependent manner, and PMBE-induced apoptosis was confirmed by DAPI staining, TUNEL assays and sub-G1 phase accumulation. Cell cycle was also arrested in G2/M phase. Immunoblotting analysis showed that cytochrome c was released, the protein expression of Bax was increased, the protein expression of Bcl-2 was down-regulated and caspase-9 and -3 were activated in PMBE-treated HeLa cells. Taken together, PMBE inhibited proliferation, induces apoptosis and causes cell cycle arrest in HeLa cells, indicating that PMBE may be a potential therapeutic agent for cancer. |
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Authors:
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Hongling Ma; Fei Lai; Heng Xie; Jinfa Wang; Hongbin Wang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Phytotherapy research : PTR Volume: 22 ISSN: 1099-1573 ISO Abbreviation: Phytother Res Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-11-04 Completed Date: 2008-12-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8904486 Medline TA: Phytother Res Country: England |
Other Details:
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Languages: eng Pagination: 1472-6 Citation Subset: IM |
Affiliation:
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The State Key Laboratory of Biocontrol and The Key Laboratory of Gene Engineering of Ministry of Education, School of Life Science, Sun Yat-sen (Zhongshan) University, Guangzhou 510275, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Caspase 3 / metabolism Caspase 9 / metabolism Cell Cycle / drug effects Cell Proliferation / drug effects Cell Survival Cytochromes c / metabolism Flow Cytometry Hela Cells Humans In Situ Nick-End Labeling Pinus / chemistry* Plant Bark / chemistry* Plant Extracts / pharmacology* Proto-Oncogene Proteins c-bcl-2 / metabolism* bcl-2-Associated X Protein / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Plant Extracts; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 9007-43-6/Cytochromes c; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9 |
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