Document Detail


Involvement of the Bcl-2 family members in Pinus massoniana bark extract induced apoptosis in HeLa cells.
MedLine Citation:
PMID:  18803251     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pinus massoniana bark extract (PMBE) contains a variety of flavonoids whose antioxidant properties have been confirmed in vitro. This study was undertaken to evaluate the cytotoxic effects and the mechanism of cell death on the PMBE-treated human cervical cancer cell line, HeLa. PMBE treatment led to cell growth inhibition in a dose- and time-dependent manner, and PMBE-induced apoptosis was confirmed by DAPI staining, TUNEL assays and sub-G1 phase accumulation. Cell cycle was also arrested in G2/M phase. Immunoblotting analysis showed that cytochrome c was released, the protein expression of Bax was increased, the protein expression of Bcl-2 was down-regulated and caspase-9 and -3 were activated in PMBE-treated HeLa cells. Taken together, PMBE inhibited proliferation, induces apoptosis and causes cell cycle arrest in HeLa cells, indicating that PMBE may be a potential therapeutic agent for cancer.
Authors:
Hongling Ma; Fei Lai; Heng Xie; Jinfa Wang; Hongbin Wang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Phytotherapy research : PTR     Volume:  22     ISSN:  1099-1573     ISO Abbreviation:  Phytother Res     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-04     Completed Date:  2008-12-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8904486     Medline TA:  Phytother Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  1472-6     Citation Subset:  IM    
Affiliation:
The State Key Laboratory of Biocontrol and The Key Laboratory of Gene Engineering of Ministry of Education, School of Life Science, Sun Yat-sen (Zhongshan) University, Guangzhou 510275, China.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*
Caspase 3 / metabolism
Caspase 9 / metabolism
Cell Cycle / drug effects
Cell Proliferation / drug effects
Cell Survival
Cytochromes c / metabolism
Flow Cytometry
Hela Cells
Humans
In Situ Nick-End Labeling
Pinus / chemistry*
Plant Bark / chemistry*
Plant Extracts / pharmacology*
Proto-Oncogene Proteins c-bcl-2 / metabolism*
bcl-2-Associated X Protein / metabolism
Chemical
Reg. No./Substance:
0/Plant Extracts; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 9007-43-6/Cytochromes c; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9

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