| Involvement of 14-3-3 proteins in the second epidermal growth factor-induced wave of Rac1 activation in the process of cell migration. | |
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MedLine Citation:
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PMID: 21868386 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Immense previous efforts have elucidated the core machinery in cell migration, actin remodeling regulated by Rho family small GTPases including RhoA, Cdc42, and Rac1; however, the spatiotemporal regulation of these molecules remains largely unknown. Here, we report that EGF induces biphasic Rac1 activation in the process of cell migration, and UTKO1, a cell migration inhibitor, inhibits the second EGF-induced wave of Rac1 activation but not the first wave. To address the regulation mechanism and role of the second wave of Rac1 activation, we identified 14-3-3ζ as a target protein of UTKO1 and also showed that UTKO1 abrogated the binding of 14-3-3ζ to Tiam1 that was responsible for the second wave of Rac1 activation, suggesting that the interaction of 14-3-3ζ with Tiam1 is involved in this event. To our knowledge, this is the first report to use a chemical genetic approach to demonstrate the mechanism of temporal activation of Rac1. |
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Authors:
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Hiroki Kobayashi; Yusuke Ogura; Masato Sawada; Ryoji Nakayama; Kei Takano; Yusuke Minato; Yasushi Takemoto; Etsu Tashiro; Hidenori Watanabe; Masaya Imoto |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-08-25 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 286 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-07 Completed Date: 2011-12-27 Revised Date: 2013-02-19 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 39259-68 Citation Subset: IM |
Affiliation:
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Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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14-3-3 Proteins
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genetics,
metabolism* Benzaldehydes / pharmacology Cell Line Cell Movement* Enzyme Activation / drug effects, genetics Epidermal Growth Factor / genetics, metabolism*, pharmacology Guanine Nucleotide Exchange Factors / genetics, metabolism Humans Protein Binding / drug effects, genetics rac1 GTP-Binding Protein / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/14-3-3 Proteins; 0/Benzaldehydes; 0/Guanine Nucleotide Exchange Factors; 0/RAC1 protein, human; 0/TIAM1 protein, human; 0/UTKO1 compound; 0/YWHAE protein, human; 62229-50-9/Epidermal Growth Factor; EC 3.6.5.2/rac1 GTP-Binding Protein |
| Comments/Corrections | |
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