Document Detail


Investigations on the influence of duodenal histidine infusion on nitrogen and amino acid turnover of growing German Holstein bulls.
MedLine Citation:
PMID:  11006833     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of a continuous duodenal infusion of L-histidine (His) (8 g/d) on the retention of nitrogen was investigated in two experiments (I, II), each of which was carried out using two young bulls. In Exps. I and II, the animals (150-250 kg BW) were fitted with a re-entrant cannula in the proximal duodenum and were fed diets containing 125 g CP/kg DM and 11.5 MJ ME/kg DM. A third experiment (III) using two young bulls (140-200 kg BW) fitted with a simple T-cannula was carried out infusing 6 g L-His. The animals were fed a low protein diet (94 g CP/kg DM and 11 MJ ME/kg DM). The study was done to find out whether or not L-His is the first limiting amino acid (AA) for growing ruminants. N retention was 28 and 31, 38 and 38, 22 and 24 g/d without L-His infusion and with L-His infusion for Exps. I, II and III, respectively. Both in the experiments with a standard protein supply (I, II) and in the experiment with reduced protein supply (III), no significant differences were found between periods with and without infusion of L-His. The utilisation of duodenal NAN varied between 39% and 50% and was also not significantly influenced by the duodenal infusion of L-His. No significant effect was observed on the flow of AA into the duodenum. The faecal excretion of AA was also not significantly influenced by the infusion of L-His. The utilisation of individual amino acids as calculated by the ratio of retained AA to intestinal apparently digested AA, did not differ significantly following the duodenal infusion of L-His. As expected, the utilisation of His decreased. Of the different essential AA, L-His was the most utilised (80%) followed by Arg (72%), Met (60%), Leu (45%) and Lys (44%), during periods without supplementation of L-His. It is concluded that the intestinal supply of L-His from the basal diet was sufficient for the potential growth level of animals under these experimental conditions. In all AA present at the proximal duodenum, L-His could have at first a limiting effect on the performance of growing young bulls with high body gain. Arg and Met, but not Lys, could be second or co-limiting AA.
Authors:
A Schoof; M Gabel; J Voigt; U Schönhusen; H Kluth
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archiv für Tierernährung     Volume:  53     ISSN:  0003-942X     ISO Abbreviation:  Arch Tierernahr     Publication Date:  2000  
Date Detail:
Created Date:  2000-10-11     Completed Date:  2000-10-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217641     Medline TA:  Arch Tierernahr     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  303-21     Citation Subset:  IM    
Affiliation:
Universität Rostock, Agrarwissenschaftliche und umweltwissenschaftliche Fakultät, Institut für umweltgerechte Tierhaltung, Germany.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism*,  physiology
Animal Feed
Animals
Catheterization / veterinary
Cattle / growth & development,  metabolism*
Diet / veterinary
Digestion / physiology
Duodenum / metabolism*
Histidine / administration & dosage*
Male
Nitrogen / metabolism*
Chemical
Reg. No./Substance:
0/Amino Acids; 71-00-1/Histidine; 7727-37-9/Nitrogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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