Document Detail

Investigations on in vitro bone resorbing activity from athymic (nude) and euthymic mouse splenic leukocytes.
MedLine Citation:
PMID:  2513857     Owner:  NLM     Status:  MEDLINE    
T-lymphocyte dependence of the production of in vitro bone resorbing activity was examined using athymic (nu/nu) and euthymic (nu/+) mouse splenic leukocytes. Conditioned medium (CM) from unstimulated splenic leukocytes of nu/nu mice had greater in vitro bone resorbing activity compared to CM from nu/+ mice (1.7- as compared to 1.2-fold increase of 45Ca release in mouse calvaria). CM from concanavalin A (Con A)-treated nu/nu and nu/+ leukocytes had 1.8-fold and no increase in 45Ca release, respectively. CM from both nu/nu and nu/+ phytohemagglutinin (PHA)-treated leukocytes had a 1.7-fold increase in 45Ca release. Bone resorbing activity from nu/nu CM was inhibited by interferon-tau (10 & 100 IU/mL) and indomethacin (2 x 10(-6) M). CM (untreated or Con A-treated) from nu/nu leukocytes had higher levels of prostaglandin E (PGE) as compared to CM from nu/+ leukocytes, and indomethacin decreased PGE levels in nu/nu CM. Leukocytes from nu/+ mice had increased mitogenesis when stimulated with PHA (1, 3, & 10 micrograms/mL) or Con A (1 and 10 micrograms/mL), whereas leukocytes from nu/nu mice were nonresponsive or had significant inhibition of mitogenesis with PHA and Con A.(ABSTRACT TRUNCATED AT 250 WORDS)
L K McCauley; T J Rosol; C C Capen; J E Horton; J Shanfeld
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Bone     Volume:  10     ISSN:  8756-3282     ISO Abbreviation:  Bone     Publication Date:  1989  
Date Detail:
Created Date:  1990-02-09     Completed Date:  1990-02-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8504048     Medline TA:  Bone     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  389-94     Citation Subset:  IM    
Department of Veterinary Pathobiology, Ohio State University, Columbus 43210.
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MeSH Terms
Animals, Newborn
Bone Resorption / immunology*
Concanavalin A / pharmacology
Culture Media
Culture Techniques
Indomethacin / pharmacology
Interferon-gamma / pharmacology
Lymphocyte Activation
Mice, Nude
Phytohemagglutinins / pharmacology
Prostaglandins E / analysis
Spleen / cytology,  immunology*
T-Lymphocytes / immunology*
Grant Support
Reg. No./Substance:
0/Culture Media; 0/Phytohemagglutinins; 0/Prostaglandins E; 11028-71-0/Concanavalin A; 53-86-1/Indomethacin; 82115-62-6/Interferon-gamma

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