| Investigations on Alternate Approach to Target Mannose Receptors on Macrophages using 4-Sulfated N-Acetyl Galactosamine more Efficiently as Compared to Mannose Decorated Liposomes : An Application in Drug Delivery. | |
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MedLine Citation:
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PMID: 21782778 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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In the present study the targeting potential of two different ligands i.e palmitoyl mannose (Man-Lip) and 4-SO(4)GalNAc (Sulf-Lip) to resident macrophages have been investigated after having decorated on the surface of Amphotericin B (AmB) loaded liposomes. In case of Sulf-Lip, the 4-SO(4)GalNAc was adsorbed through electrostatic interaction on cationic liposomes, which was confirmed by change in zeta potential from +48.2 ± 3.7 mV for Lip to +12.2 ± 1.3 mV for Sulf-Lip. The mean particle size of Sulf-Lip and Man-Lip was found to be 139.4 ± 7.4 nm and 147.4 ± 8.6 nm respectively. Flow cytometric data reveals enhanced uptake of Sulf-Lip in both J774 and RAW cell lines as compared to Man-Lip. Intracellular localization studies indicate that the fluorescence intensity of Sulf-Lip was much higher as compared to Man-Lip and Lip formulations. Sulf-Lip and Man-Lip showed significantly higher localization of AmB at all time points compared to Lip (P < .05) after i.v. administration. The studies provides evidences that 4-SO(4)GalNAc possess promising feature for targeting resident macrophages and its application in the conditions of leishmaniasis is in offing. |
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Authors:
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Deepak Singodia; Ashwni Verma; Rahul K Verma; Prabhat Ranjan Mishra |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-7-20 |
Journal Detail:
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Title: Nanomedicine : nanotechnology, biology, and medicine Volume: - ISSN: 1549-9642 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-7-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101233142 Medline TA: Nanomedicine Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier Inc. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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