Document Detail

Investigation of transmembrane protein fused in lipid bilayer membranes supported on porous silicon.
MedLine Citation:
PMID:  23276154     Owner:  NLM     Status:  In-Data-Review    
This article investigates a device made from a porous silicon structure supporting a lipid bilayer membrane (LBM)fused with Epithelial Sodium Channel protein. The electrochemically-fabricated porous silicon template had pore diameters in the range 0.2~2 µm. Membranes were composed of two synthetic phospholipids: 1,2-diphytanoyl-sn-glycero-3-phosphoserine and 1,2-diphytanoyl-sn-glycero-3-phosphoethanolamine. The LBMwas formed by means of the Langmuir-Blodgett and Langmuir-Schaefer techniques, at a monolayer surface tension of 26 m Nm(-1) in room temperature and on a deionized water subphase, which resulted in an average molecular area of 0.68-0.73 nm(2). Fusion of transmembrane protein was investigated using Atomic Force Microscopy. Initial atomic force microscopy results demonstrate the ability to support lipid bilayers fused with transmembrane proteins across a porous silicon substrate. However, more control of the membrane's surface tension using traditional membrane fusion techniques is required to optimize protein incorporation.
Khalid Hasan Tantawi; Ramon Cerro; Bakhrom Berdiev; M Elena Diaz Martin; Francisco Javier Montes; Darayas Patel; John D Williams
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of medical engineering & technology     Volume:  37     ISSN:  1464-522X     ISO Abbreviation:  J Med Eng Technol     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7702125     Medline TA:  J Med Eng Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  28-34     Citation Subset:  IM    
Department of Electrical and Computer Engineering.
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