Document Detail


Investigation of transmembrane protein fused in lipid bilayer membranes supported on porous silicon.
MedLine Citation:
PMID:  23276154     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
This article investigates a device made from a porous silicon structure supporting a lipid bilayer membrane (LBM)fused with Epithelial Sodium Channel protein. The electrochemically-fabricated porous silicon template had pore diameters in the range 0.2~2 µm. Membranes were composed of two synthetic phospholipids: 1,2-diphytanoyl-sn-glycero-3-phosphoserine and 1,2-diphytanoyl-sn-glycero-3-phosphoethanolamine. The LBMwas formed by means of the Langmuir-Blodgett and Langmuir-Schaefer techniques, at a monolayer surface tension of 26 m Nm(-1) in room temperature and on a deionized water subphase, which resulted in an average molecular area of 0.68-0.73 nm(2). Fusion of transmembrane protein was investigated using Atomic Force Microscopy. Initial atomic force microscopy results demonstrate the ability to support lipid bilayers fused with transmembrane proteins across a porous silicon substrate. However, more control of the membrane's surface tension using traditional membrane fusion techniques is required to optimize protein incorporation.
Authors:
Khalid Hasan Tantawi; Ramon Cerro; Bakhrom Berdiev; M Elena Diaz Martin; Francisco Javier Montes; Darayas Patel; John D Williams
Related Documents :
23218354 - Milling of rice grains: effects of starch/flour structures on gelatinization and pastin...
23030044 - Molecular dynamics simulations to examine structure, energetics and evaporation/condens...
24598074 - Role of the rho gtpase rac in the activation of the phagocyte nadph oxidase: outsourcin...
24468064 - Multi-step formation of a hemifusion diaphragm for vesicle fusion revealed by all-atom ...
20030324 - Differentiating oxicam nonsteroidal anti-inflammatory drugs in phosphoglyceride monolay...
6796114 - Electrostatic influence of local cysteine environments on disulfide exchange kinetics.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of medical engineering & technology     Volume:  37     ISSN:  1464-522X     ISO Abbreviation:  J Med Eng Technol     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7702125     Medline TA:  J Med Eng Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  28-34     Citation Subset:  IM    
Affiliation:
Department of Electrical and Computer Engineering.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Contribution of Antibody-based Protein Profiling to the Human Chromosome-centric Proteome Project (C...
Next Document:  An implementation of a CBIR system based on SVM learning scheme.