Document Detail


Investigation of a synthetic peptide as immunogen for a variant epidermal growth factor receptor associated with gliomas.
MedLine Citation:
PMID:  8360327     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have previously demonstrated antibody production to a glioma-associated variant form of the human epidermal growth factor receptor in rabbits that had received a synthetic peptide mimicking the unique primary structure of the variant protein as immunogen. We report here the response of mice, rabbits, goats, and macaques immunized by various protocols to this peptide. Titers to both peptide- and cell-elaborated variant receptor were measured, and the capacity to recognize the variant receptor in human tumor samples was determined. Within the range of species and strains investigated, we demonstrated a variable species-associated response to the peptide (rabbits > mice > goats > rats > macaques). Rabbits and a single goat produced specific, high titer antibody activity to the variant receptor protein following immunization with peptide alone. Murine titers to the parent protein were not appreciable following peptide immunization alone; additional immunization with variant receptor as expressed on cell membranes was used to boost this response.
Authors:
C J Wikstrand; S D Stanley; P A Humphrey; C N Pegram; G E Archer; S Kurpad; M Shibuya; D D Bigner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neuroimmunology     Volume:  46     ISSN:  0165-5728     ISO Abbreviation:  J. Neuroimmunol.     Publication Date:  1993 Jul 
Date Detail:
Created Date:  1993-09-28     Completed Date:  1993-09-28     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8109498     Medline TA:  J Neuroimmunol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  165-73     Citation Subset:  IM    
Affiliation:
Department of Pathology, Duke University Medical Center, Durham, NC 27710.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies, Monoclonal / immunology
Autoantigens / immunology
Glioma / immunology*
Goats
Macaca
Mice
Molecular Sequence Data
Peptides / chemistry,  immunology
Rabbits
Receptor, Epidermal Growth Factor / immunology*
Grant Support
ID/Acronym/Agency:
CA11898/CA/NCI NIH HHS; NS20023/NS/NINDS NIH HHS; NS29955/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Autoantigens; 0/Peptides; EC 2.7.10.1/Receptor, Epidermal Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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