| Investigation of a synthetic peptide as immunogen for a variant epidermal growth factor receptor associated with gliomas. | |
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MedLine Citation:
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PMID: 8360327 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously demonstrated antibody production to a glioma-associated variant form of the human epidermal growth factor receptor in rabbits that had received a synthetic peptide mimicking the unique primary structure of the variant protein as immunogen. We report here the response of mice, rabbits, goats, and macaques immunized by various protocols to this peptide. Titers to both peptide- and cell-elaborated variant receptor were measured, and the capacity to recognize the variant receptor in human tumor samples was determined. Within the range of species and strains investigated, we demonstrated a variable species-associated response to the peptide (rabbits > mice > goats > rats > macaques). Rabbits and a single goat produced specific, high titer antibody activity to the variant receptor protein following immunization with peptide alone. Murine titers to the parent protein were not appreciable following peptide immunization alone; additional immunization with variant receptor as expressed on cell membranes was used to boost this response. |
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Authors:
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C J Wikstrand; S D Stanley; P A Humphrey; C N Pegram; G E Archer; S Kurpad; M Shibuya; D D Bigner |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of neuroimmunology Volume: 46 ISSN: 0165-5728 ISO Abbreviation: J. Neuroimmunol. Publication Date: 1993 Jul |
Date Detail:
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Created Date: 1993-09-28 Completed Date: 1993-09-28 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8109498 Medline TA: J Neuroimmunol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 165-73 Citation Subset: IM |
Affiliation:
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Department of Pathology, Duke University Medical Center, Durham, NC 27710. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Antibodies, Monoclonal / immunology Autoantigens / immunology Glioma / immunology* Goats Macaca Mice Molecular Sequence Data Peptides / chemistry, immunology Rabbits Receptor, Epidermal Growth Factor / immunology* |
| Grant Support | |
ID/Acronym/Agency:
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CA11898/CA/NCI NIH HHS; NS20023/NS/NINDS NIH HHS; NS29955/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Autoantigens; 0/Peptides; EC 2.7.10.1/Receptor, Epidermal Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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