| Investigation of surface plasmon resonance biosensor for skin sensitizers studies. | |
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MedLine Citation:
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PMID: 19073248 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Non-animal testing methods are a current challenge in terms of the assessment of skin sensitization potential for new chemicals. Our objective was to investigate a surface plasmon resonance (SPR) biosensor to screen allergens against nucleophilic amino acids (cysteine, lysine and histidine) in a direct binding assay. Amino acids were immobilized on the sensor surface and exposed to different skin allergens (chemicals and fragrances) with varying sensitizing potential. Cysteine was found to be more reactive than lysine while histidine showed the lowest reactivity. The interactions observed were different depending on the allergen/amino acids involved. It appeared that weak allergens could quickly dissociate from the ligand, whereas strong and extreme allergens remained bound to the amino acids. The SPR report points allowed a good discrimination of the tested allergens. With this technology, we can observe low energy bindings and get information on the stability of the hapten/amino acid complex which seem relevant for the determination of skin sensitization potential. This prospective experiment showed the potential of real-time SPR to generate specific report points to refine the skin sensitization allergen assessment. |
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Authors:
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Christine Achilleos; Magalie Tailhardat; Pascal Courtellemont; Béatrice Le Varlet; Didier Dupont |
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Publication Detail:
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Type: In Vitro; Journal Article Date: 2008-12-03 |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 23 ISSN: 0887-2333 ISO Abbreviation: Toxicol In Vitro Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-02-09 Completed Date: 2009-04-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
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Languages: eng Pagination: 308-18 Citation Subset: IM |
Affiliation:
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INRA, UR342 Technologie et Analyses Laitières, BP 20089, F-39800 Poligny, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Allergens
/
chemistry*,
classification,
toxicity Amino Acids / chemistry* Animal Testing Alternatives* Dermatitis, Contact / etiology Drug Hypersensitivity / etiology Haptens / chemistry Local Lymph Node Assay Protein Binding Skin Tests Surface Plasmon Resonance / methods* |
| Chemical | |
Reg. No./Substance:
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0/Allergens; 0/Amino Acids; 0/Haptens |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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