| Investigation of subcellular acidic compartments using alpha-aminophosphonate 31P nuclear magnetic resonance probes. | |
| | |
MedLine Citation:
|
PMID: 18585363 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The 31P nuclear magnetic resonance (NMR) characteristics, toxicity, and cellular penetration of five linear or cyclic alpha-aminophosphonate highly sensitive pH probes were investigated in Dictyostelium discoideum cells and isolated rat hearts and were compared with three phosphonic acid derivatives. The line width broadening at pH approximately pK(a), which was satisfactorily modelized for all compounds, was significantly limited in biological milieu for the new markers, affording a four- to sixfold better accuracy in pH determination. Cellular uptake or washout of nontoxic concentrations (< 15 mM) of alpha-aminophosphonates occurred by rapid passive permeation, whereas standard probes required a much slower fluid-phase pinocytosis and transport processes that could ultimately lead to trapping. Using mild concentrations (< 4 mM) three alpha-aminophosphonates having 6 < pK(a) < 7 allowed an easy and simultaneous 31P NMR determination of cytosolic, acidic, and extracellular compartments in anoxic-reoxygenated or starving D. discoideum. |
| | |
Authors:
|
Gaëlle Gosset; Michel Satre; Bruno Blaive; Jean-Louis Clément; Jean-Baptiste Martin; Marcel Culcasi; Sylvia Pietri |
Related Documents
:
|
10606153 - Growth and survival of various strains of enterohemorrhagic escherichia coli in hydroch... 7296403 - Germination of macroconidia and growth of sporidesmium sclerotivorum in vitro. 16867763 - Determination of water in penicillins using fast karl fischer reagents and electronic e... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-06-05 |
Journal Detail:
|
Title: Analytical biochemistry Volume: 380 ISSN: 1096-0309 ISO Abbreviation: Anal. Biochem. Publication Date: 2008 Sep |
Date Detail:
|
Created Date: 2008-07-30 Completed Date: 2008-09-23 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: United States |
Other Details:
|
Languages: eng Pagination: 184-94 Citation Subset: IM |
Affiliation:
|
Groupe Sondes Moléculaires en Biologie, Laboratoire Chimie Provence-UMR 6264, Universités d'Aix-Marseille-CNRS, F-13397 Marseille Cedex 20, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amines
/
chemistry*,
pharmacokinetics,
toxicity Animals Cells, Cultured Dictyostelium / chemistry, drug effects, metabolism Heart / drug effects Hydrogen-Ion Concentration Myocardium / chemistry, metabolism Neuroglia / chemistry, drug effects, metabolism Nuclear Magnetic Resonance, Biomolecular / methods* Permeability Phosphonic Acids / chemistry*, pharmacokinetics, toxicity Phosphorus Isotopes / analysis Rats Subcellular Fractions / chemistry, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Amines; 0/Phosphonic Acids; 0/Phosphorus Isotopes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Conjugated autoxidizable triene (CAT) assay: a novel spectrophotometric method for determination of ...
Next Document: Dimethyl sulfoxide-induced hydroxyapatite formation: a biological model of matrix vesicle nucleation...