| Investigation on strains of Legionella pneumophila, isolated from a hospital of Milano, with three genotyping methods | |
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MedLine Citation:
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PMID: 20058542 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Various techniques have been developed in recent years for the molecular typing of microorganisms. Remains particularly difficult to isolate clinical strains for the low availability of cases and even more problematic matching clinical / environmental strains. We investigated 13 strains of Legionella pneumophila of clinical and environmental origin, isolated in 3 Health Facilities in Milan (2003-2006), using three molecular typing methods: Pulse-Field Gel Electrophoresis, Amplified Fragment Length Polymorphism and Sequence-Based Typing. PFGE and AFLP showed the correlation between a clinical case with only one of the environmental isolates taken from the places frequented by the patient, demonstrating with certainty the nosocomial origin of the case and identifying the source of infection in the shower water (Clin. 1 and Env. 1N). Two clinical samples from patients admitted to different wards presented an identical profile, which suggests that the nosocomial origin assumed an epidemic form, even without having isolated the environmental strain due to the absence of samples drawn during the period under consideration (Clin. 2 and 3). Finally, the comparison between the isolated environmental strains demonstrated a heterogeneous presence of strains, not correlated to each other although they belong to the same serum-group, having profiles that are clearly different regarding number and position of bands (Env. 2 and 4). The profile 2,10,18,10,1,1 had never been isolated and typed previously in Europe. The SBT has proved a better technique for reproducibility and interpretation of results than PFGE and AFLP To complete studies on SBT method, now considered gold standard, is currently being the EWGLI 5th Proficiency Panel, in which we are actively involved with the genotyping of five strains according to the latest version of the protocol (4.1). |
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Authors:
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A Bianchi; M Tesauro; M Consonni; M G Galli |
Publication Detail:
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Type: English Abstract; Journal Article |
Journal Detail:
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Title: Annali di igiene : medicina preventiva e di comunità Volume: 21 ISSN: 1120-9135 ISO Abbreviation: Ann Ig Publication Date: 2009 Sep-Oct |
Date Detail:
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Created Date: 2010-01-11 Completed Date: 2010-01-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9002865 Medline TA: Ann Ig Country: Italy |
Other Details:
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Languages: ita Pagination: 517-22 Citation Subset: IM |
Affiliation:
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Dipartimento di Sanità Pubblica, Microbiologia e Virologia, Università degli Studi di Milano, Milano. annalisa.bianchi@unimi.it |
Vernacular Title:
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Genotipizzazione di ceppi di Legionella pneumophila, isolati in strutture sanitarie di differenti tipologie. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amplified Fragment Length Polymorphism Analysis
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methods Bacterial Typing Techniques / methods* Cross Infection / diagnosis*, epidemiology Electrophoresis, Gel, Pulsed-Field / methods Humans Italy / epidemiology Legionella pneumophila / classification*, genetics, isolation & purification* Legionnaires' Disease / diagnosis*, epidemiology Predictive Value of Tests Reproducibility of Results Retrospective Studies |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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