Document Detail


Investigation of stable and transient protein-protein interactions: Past, present, and future.
MedLine Citation:
PMID:  23193082     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This article presents an overview of the literature and a review of recent advances in the analysis of stable and transient protein-protein interactions (PPIs) with a focus on their function within cells, organs, and organisms. The significance of PTMs within the PPIs is also discussed. We focus on methods to study PPIs and methods of detecting PPIs, with particular emphasis on electrophoresis-based and MS-based investigation of PPIs, including specific examples. The validation of PPIs is emphasized and the limitations of the current methods for studying stable and transient PPIs are discussed. Perspectives regarding PPIs, with focus on bioinformatics and transient PPIs are also provided.
Authors:
Armand G Ngounou Wetie; Izabela Sokolowska; Alisa G Woods; Urmi Roy; Joseph A Loo; Costel C Darie
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Review     Date:  2013-01-18
Journal Detail:
Title:  Proteomics     Volume:  13     ISSN:  1615-9861     ISO Abbreviation:  Proteomics     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-18     Completed Date:  2013-07-31     Revised Date:  2014-02-04    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  538-57     Citation Subset:  IM    
Copyright Information:
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, Affinity
Humans
Multiprotein Complexes / isolation & purification,  metabolism
Protein Binding
Protein Interaction Mapping / methods*
Protein Processing, Post-Translational
Proteome / isolation & purification,  metabolism*
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
Grant Support
ID/Acronym/Agency:
GM103479/GM/NIGMS NIH HHS; R01 GM103479/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Multiprotein Complexes; 0/Proteome
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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