Document Detail


Investigation of some antimicrobial procedures on the in vitro development of early murine embryos aimed toward developing methods for the disinfection of mammalian embryos prior to transfer.
MedLine Citation:
PMID:  1826723     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Eight-cell, zona pellucida-intact mouse embryos were exposed to the following substances or procedures that have been reported to have germicidal effects to determine if the embryos would survive and develop under in vitro conditions: the photosensitive substances hematoporphyrin, hematoporphyrin derivative, 8-methoxypsoralen, 4,5',8-trimethylpsoralen, and thiopyronine; the enzymes lipase (0.5%), phospholipase C (2 U/ml), chymotrypsin (0.5%), and trypsin (0.5%); pH 5.0; and helium/neon laser light, visible light, ultraviolet A light, and ultraviolet C light. Under the conditions used, embryos were not adversely affected by hematoporphyrin and/or helium/neon laser light; methoxypsoralen and/or ultraviolet A light; lipase; trypsin; pH 5.0 for 20 min; and visible light. Variable results were obtained from hematoporphyrin derivative with laser light. Thiopyronine, trimethylpsoralen in combination with ultraviolet A light, and ultraviolet C light killed embryos, and chymotrypsin and phospholipase C were harmful at 10- and 15-min exposure times, respectively.
Authors:
A Bielanski; W C Hare
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of in vitro fertilization and embryo transfer : IVF     Volume:  8     ISSN:  0740-7769     ISO Abbreviation:  J In Vitro Fert Embryo Transf     Publication Date:  1991 Feb 
Date Detail:
Created Date:  1991-05-22     Completed Date:  1991-05-22     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8412594     Medline TA:  J In Vitro Fert Embryo Transf     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  24-32     Citation Subset:  IM    
Affiliation:
Animal Diseases Research Institute, Agriculture Canada, Nepean, Ontario.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Survival / drug effects,  radiation effects
Chymotrypsin / therapeutic use
Disinfection / methods*
Dose-Response Relationship, Drug
Dose-Response Relationship, Radiation
Embryo Transfer
Embryo, Mammalian* / drug effects,  radiation effects
Female
Helium
Hematoporphyrin Derivative
Hematoporphyrins / therapeutic use
Hydrogen-Ion Concentration
Laser Therapy
Lipase / therapeutic use
Methoxsalen / therapeutic use
Mice
Neon
Pyronine / analogs & derivatives,  therapeutic use
Radiation-Sensitizing Agents
Trioxsalen / therapeutic use
Trypsin / therapeutic use
Type C Phospholipases / therapeutic use
Ultraviolet Rays
Chemical
Reg. No./Substance:
0/Hematoporphyrins; 0/Radiation-Sensitizing Agents; 2412-14-8/thiopyronine; 298-81-7/Methoxsalen; 3902-71-4/Trioxsalen; 68335-15-9/Hematoporphyrin Derivative; 7440-01-9/Neon; 7440-59-7/Helium; 92-32-0/Pyronine; EC 3.1.1.3/Lipase; EC 3.1.4.-/Type C Phospholipases; EC 3.4.21.1/Chymotrypsin; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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