Document Detail


Investigation of serotonin type 4 receptor expression in human and non-human primate gastrointestinal samples.
MedLine Citation:
PMID:  16894306     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The serotonin type 4 (5-HT4) receptor has been associated with functions of the gastrointestinal tract such as modulation of the peristaltic reflex, smooth muscle tone, intestinal secretion and visceral sensitivity. The activation of peripheral 5-HT4 receptors with agonists such as tegaserod has been shown to accelerate gastric emptying and improve symptoms of constipation in animals and humans. However, detailed data on the expression profile and on the localization of this receptor subtype are lacking so far. OBJECTIVE: To study the pattern and expression levels of 5-HT4 receptor messenger RNA expression in the gut. METHOD: Normal tissue samples were collected from the whole gastrointestinal tract of patients undergoing abdominal surgery and, in addition, of monkeys. We performed a comprehensive analysis of 5-HT4 receptor expression by quantitative reverse transcription-polymerase chain reaction, using human and non-human primate tissues from the oesophagus to the rectum. In addition, the brain and heart of non-human primates were analysed. RESULTS: Significantly higher levels of 5-HT4 receptor mRNA were measured in the human stomach, duodenum, jejunum, ileum and caecum and also in the corresponding non-human primate gut segments, ranging from 2- to 12-fold compared with the liver. No differences were found between females and males of both human and non-human primates. CONCLUSIONS: These results show 5-HT4 receptor mRNA expression throughout the gastrointestinal tract in humans and primates, and also support the preclinical and clinical findings of 5-HT4 receptors ligands exhibiting multiple effects throughout the gastrointestinal tract.
Authors:
Robert Mader; Thomas Kocher; Joerg Haier; Grazyna Wieczorek; Hans-Juergen Pfannkuche; Moriko Ito
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Publication Detail:
Type:  Journal Article; Multicenter Study    
Journal Detail:
Title:  European journal of gastroenterology & hepatology     Volume:  18     ISSN:  0954-691X     ISO Abbreviation:  Eur J Gastroenterol Hepatol     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-08     Completed Date:  2007-07-26     Revised Date:  2009-10-16    
Medline Journal Info:
Nlm Unique ID:  9000874     Medline TA:  Eur J Gastroenterol Hepatol     Country:  England    
Other Details:
Languages:  eng     Pagination:  945-50     Citation Subset:  IM    
Affiliation:
Novartis Institutes for BioMedical Research, GI Department, Basel, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Female
Gastrointestinal Tract / chemistry*
Humans
Male
Primates
RNA, Messenger / analysis
Receptors, Serotonin, 5-HT4 / analysis*
Reverse Transcriptase Polymerase Chain Reaction
Sex Factors
Chemical
Reg. No./Substance:
0/RNA, Messenger; 158165-40-3/Receptors, Serotonin, 5-HT4

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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