Document Detail

Investigation of the selenium metabolism in cancer cell lines.
MedLine Citation:
PMID:  21161099     Owner:  NLM     Status:  Publisher    
The aim of this work was to compare different selenium species for their ability to induce cell death in different cancer cell lines, while investigating the underlying chemistry by speciation analysis. A prostate cancer cell line (PC-3), a colon cancer cell line (HT-29) and a leukaemia cell line (Jurkat E6-1) were incubated with five selenium compounds representing inorganic as well as organic Se compounds in different oxidation states. Selenomethionine (SeMet), Se-methylselenocysteine (MeSeCys), methylseleninic acid (MeSeA), selenite and selenate in the concentration range 5-100 μM were incubated with cells for 24 h and the induction of cell death was measured using flow cytometry. The amounts of total selenium in cell medium, cell lysate and the insoluble fractions was determined by ICP-MS. Speciation analysis of cellular fractions was performed by reversed phase, anion exchange and size exclusion chromatography and ICP-MS detection. The selenium compounds exhibited large differences in their ability to induce cell death in the three cell lines and the susceptibilities of the cell lines were different. Full recovery of selenium in the cellular fractions was observed for all Se compounds except MeSeA. Speciation analysis showed that MeSeA was completely transformed during the incubations, while metabolic conversion of the other Se compounds was limited. Production of volatile dimethyl diselenide was observed for MeSeA and MeSeCys. MeSeA, MeSeCys and selenite showed noticeable protein binding. Correlations between cell death induction and the Se compounds transformations could not be demonstrated.
Kristoffer Lunøe; Charlotte Gabel-Jensen; Stefan Stürup; Lars Andresen; Søren Skov; Bente Gammelgaard
Related Documents :
25380639 - Microwave-mediated extracellular synthesis of metallic silver and zinc oxide nanopartic...
19854939 - Bionumbers--the database of key numbers in molecular and cell biology.
25365589 - Is sphere assay useful for the identification of cancer initiating cells of the ovary?
21556579 - Changes in expression of neural cell-adhesion molecule ard desmoplakin associated with ...
21140429 - Transformation of rat intestinal epithelial cells by over-expression of rab25 is microt...
25319389 - Marine sponge cribrochalina vasculum compounds activate intrinsic apoptotic signaling a...
4358429 - Inhibition of cell growth in the g1 phase by adenosine 3', 5'-cyclic monophosphate.
24682729 - Combined treatment with quercetin and imperatorin as a potent strategy for killing hela...
19192169 - Newcastle disease virus represses the activation of human hepatic stellate cells and re...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-12-16
Journal Detail:
Title:  Metallomics : integrated biometal science     Volume:  -     ISSN:  1756-591X     ISO Abbreviation:  -     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101478346     Medline TA:  Metallomics     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Dual catalysis: combining photoredox and Lewis base catalysis for direct Mannich reactions.
Next Document:  Small-sized silicon nanoparticles: new nanolights and nanocatalysts.