Document Detail


Investigation of saturation and photobleaching of allophycocyanin by single-molecule recrossing events.
MedLine Citation:
PMID:  20223069     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phycobiliprotein fluorescent labels are playing an increasingly important role in bioanalysis. They are also being used more and more frequently as light-harvesting materials for energy research. It is therefore critical to study the working conditions of these fluorescent dyes. Allophycocyanin (APC) belongs to a group of phycobiliproteins and features red excitation and emission, making it both a useful fluorophore and light-harvesting material. Saturation irradiance and photobleaching of APC were studied by single-molecule detection in this work. The mean fluorescence intensity at different laser powers was calculated from extracted single-molecule fluorescence peaks. By interpolating the figure of the mean fluorescence intensity as a function of excitation power, the experimental saturation irradiance can be extracted. By comparing the experimental with the calculated saturation irradiance, it can be demonstrated that the triplet state for APC was formed at higher excitation irradiance. The technique of molecular recrossing events was applied to investigate the photobleaching of APC. Normalized recrossing events confirmed that photobleaching occurred at high excitation power. This work provided the optimizing experimental conditions for APC both as a fluorophore and as a light-harvesting molecule.
Authors:
Yu Tian; Dimitri Pappas
Related Documents :
17270459 - Parallel changes in non-photochemical quenching properties, photosynthesis and d1 level...
2105069 - Photodeactivation of ethyl violet: a potential hazard of sodasorb.
863749 - A microfluorescent pas method for the quantitative demonstration of cytoplasmic 1,2-gly...
21068989 - Confocal theta fluorescence microscopy with annular apertures.
12231769 - Effects of anaerobiosis on chlorophyll fluorescence yield in spinach (spinacia oleracea...
25027769 - Assessment of visual fixation in vegetative and minimally conscious states.
836249 - The cardiovascular response to acoustic stimuli.
6643289 - Responses of gerbil and guinea pig auditory nerve fibers to low-frequency sinusoids.
18582849 - Cannabinoids and hamster circadian activity rhythms.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied spectroscopy     Volume:  64     ISSN:  1943-3530     ISO Abbreviation:  Appl Spectrosc     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-12     Completed Date:  2010-06-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372406     Medline TA:  Appl Spectrosc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  324-7     Citation Subset:  IM    
Affiliation:
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas 79409-1061, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Fluorescent Dyes / chemistry
Models, Molecular
Photobleaching*
Phycocyanin / chemistry*
Spectrum Analysis / methods*
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/allophycocyanin; 11016-15-2/Phycocyanin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A new method for determination of self-absorption coefficients of emission lines in laser-induced br...
Next Document:  The characterization of phospholipid functional group probe species on respirable silicon-containing...