Document Detail

Investigation of radical-based damage of RNase A in aqueous solution and lipid vesicles.
MedLine Citation:
PMID:  16177964     Owner:  NLM     Status:  MEDLINE    
The gamma-irradiation of bovine pancreatic ribonuclease A (RNase A) in aqueous solution were investigated at different doses by vibrational spectroscopy as well as enzymatic assay, electrophoresis, and HPLC analysis. Both functional and structural changes of the protein were caused by attack of H(*) atoms and (*)OH radicals. In particular, Raman spectroscopy was shown to be a useful tool in identifying conformational changes of the protein structure and amino acidic residues that are preferential sites of the radical attack (i.e., tyrosine and methionine). After partial structural changes by the initial radical attack, the internal sulfur-containing amino acid residues were rendered susceptible to transformation. By using the biomimetic model of dioleoyl phosphatidyl choline vesicle suspensions containing RNase A, the damage to methione residues could be connected to a parallel alteration of membrane unsaturated lipids. In fact, thiyl radical species formed from protein degradation can diffuse into the lipid bilayer and cause isomerization of the naturally occurring cis double bonds. As a consequence, trans unsaturated fatty acids are formed in vesicles and can be considered to be markers of this protein damage.
Armida Torreggiani; Maurizio Tamba; Immacolata Manco; M R Faraone-Mennella; Carla Ferreri; C Chatgilialoglu
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Biopolymers     Volume:  81     ISSN:  0006-3525     ISO Abbreviation:  Biopolymers     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-12-05     Completed Date:  2006-01-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372525     Medline TA:  Biopolymers     Country:  United States    
Other Details:
Languages:  eng     Pagination:  39-50     Citation Subset:  IM    
Copyright Information:
Copyright 2005 Wiley Periodicals, Inc.
Istituto ISOF, Consiglio Nazionale delle Ricerche, Bologna, Italy.
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MeSH Terms
Chromatography, High Pressure Liquid
Dose-Response Relationship, Radiation
Electrophoresis, Polyacrylamide Gel
Free Radicals / chemistry*
Gamma Rays
Liposomes / chemistry*
Models, Chemical
Phosphatidylcholines / chemistry
Protein Conformation
Protein Structure, Secondary
Ribonuclease, Pancreatic / analysis,  chemistry*
Spectrum Analysis, Raman
Time Factors
Water / chemistry*
Reg. No./Substance:
0/Free Radicals; 0/Liposomes; 0/Phosphatidylcholines; 10015-85-7/1,2-oleoylphosphatidylcholine; 7732-18-5/Water; EC, Pancreatic

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