| Investigation of radical-based damage of RNase A in aqueous solution and lipid vesicles. | |
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MedLine Citation:
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PMID: 16177964 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The gamma-irradiation of bovine pancreatic ribonuclease A (RNase A) in aqueous solution were investigated at different doses by vibrational spectroscopy as well as enzymatic assay, electrophoresis, and HPLC analysis. Both functional and structural changes of the protein were caused by attack of H(*) atoms and (*)OH radicals. In particular, Raman spectroscopy was shown to be a useful tool in identifying conformational changes of the protein structure and amino acidic residues that are preferential sites of the radical attack (i.e., tyrosine and methionine). After partial structural changes by the initial radical attack, the internal sulfur-containing amino acid residues were rendered susceptible to transformation. By using the biomimetic model of dioleoyl phosphatidyl choline vesicle suspensions containing RNase A, the damage to methione residues could be connected to a parallel alteration of membrane unsaturated lipids. In fact, thiyl radical species formed from protein degradation can diffuse into the lipid bilayer and cause isomerization of the naturally occurring cis double bonds. As a consequence, trans unsaturated fatty acids are formed in vesicles and can be considered to be markers of this protein damage. |
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Authors:
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Armida Torreggiani; Maurizio Tamba; Immacolata Manco; M R Faraone-Mennella; Carla Ferreri; C Chatgilialoglu |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Biopolymers Volume: 81 ISSN: 0006-3525 ISO Abbreviation: Biopolymers Publication Date: 2006 Jan |
Date Detail:
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Created Date: 2005-12-05 Completed Date: 2006-01-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372525 Medline TA: Biopolymers Country: United States |
Other Details:
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Languages: eng Pagination: 39-50 Citation Subset: IM |
Copyright Information:
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Copyright 2005 Wiley Periodicals, Inc. |
Affiliation:
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Istituto ISOF, Consiglio Nazionale delle Ricerche, Bologna, Italy. torreggiani@isof.cnr.it |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Chromatography, High Pressure Liquid Dose-Response Relationship, Radiation Electrophoresis, Polyacrylamide Gel Free Radicals / chemistry* Gamma Rays Isomerism Liposomes / chemistry* Models, Chemical Phosphatidylcholines / chemistry Protein Conformation Protein Structure, Secondary Ribonuclease, Pancreatic / analysis, chemistry* Spectrum Analysis, Raman Time Factors Water / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Free Radicals; 0/Liposomes; 0/Phosphatidylcholines; 10015-85-7/1,2-oleoylphosphatidylcholine; 7732-18-5/Water; EC 3.1.27.5/Ribonuclease, Pancreatic |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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