Document Detail

Investigation of a pulsed-laser thermo-optical absorbance detector for the determination of food preservatives separated by capillary electrophoresis.
MedLine Citation:
PMID:  8876438     Owner:  NLM     Status:  MEDLINE    
Thermo-optical absorbance (TOA) detection using a KrF excimer waveguide laser for detection of benzoic acid, dehydroacetic acid and sorbic acid separated by capillary electrophoresis (CE) was studied. Detection limits were, on average, ten times better than those for on-column UV absorbance methods with CE, and two or more times better than those for UV absorbance with HPLC. The influence of increased laser power on TOA detection sensitivity was found to be strong for benzoic and dehydroacetic acids but quite weak for sorbic acid. It was discovered that photoisomerization of sorbic acid (2,4-hexadienoic acid) occurred readily in the detection volume at moderate laser powers (P(ave) = 3 mW) and increased with slow electroosmotic flows (< 6 cm/min). The TOA method described here shows improved detection sensitivity for CE analyses of compounds having only weak absorptivities (< 5% of maximum) at lambda = 248 nm, and thus demonstrates its utility for determination of a variety of analytes in a single separation.
K C Waldron; J Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. B, Biomedical applications     Volume:  683     ISSN:  1572-6495     ISO Abbreviation:  J. Chromatogr. B, Biomed. Appl.     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1997-03-05     Completed Date:  1997-03-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9421796     Medline TA:  J Chromatogr B Biomed Appl     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  47-54     Citation Subset:  IM    
Département de Chimie, Université de Montréal, Québec, Canada.
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MeSH Terms
Electrophoresis, Capillary
Food Preservatives / analysis*,  isolation & purification
Optics and Photonics
Sensitivity and Specificity
Spectrum Analysis / methods*
Reg. No./Substance:
0/Food Preservatives

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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