| Investigation of a potential scintigraphic tracer for imaging apoptosis: radioiodinated annexin v-kunitz protease inhibitor fusion protein. | |
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MedLine Citation:
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PMID: 21584284 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Radiolabeled annexin V (ANV) has been widely used for imaging cell apoptosis. Recently, a novel ANV-Kunitz-type protease inhibitor fusion protein, ANV-6L15, was found to be a promising probe for improved apoptosis detection based on its higher affinity to phosphatidylserine (PS) compared to native ANV. The present paper investigates the feasibility of apoptosis detection using radioiodinated ANV-6L15. Native ANV and ANV-6L15 were labeled with iodine-123 and iodine-125 using Iodogen method. The binding between the radioiodinated proteins and erythrocyte ghosts or chemical-induced apoptotic cells was examined. ANV-6L15 can be radioiodinated with high yield (40%-60%) and excellent radiochemical purity (>95%). (123)I-ANV-6L15 exhibited a higher binding ratio to erythrocyte ghosts and apoptotic cells compared to (123)I-ANV. The biodistribution of (123)I-ANV-6L15 in mice was also characterized. (123)I-ANV-6L15 was rapidly cleared from the blood. High uptake in the liver and the kidneys may limit the evaluation of apoptosis in abdominal regions. Our data suggest that radiolabled ANV-6L15 may be a better scintigraphic tracer than native ANV for apoptosis detection. |
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Authors:
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Mei-Hsiu Liao; Tong-Rong Jan; Chao-Chih Chiang; Kuo-Chen Yen; Tse-Zung Liao; Ming-Wei Chen; Chin-Wen Chi; Tze-Chein Wun; Tzu-Chen Yen; Shiaw-Pyng Wey |
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Publication Detail:
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Type: Journal Article Date: 2011-04-27 |
Journal Detail:
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Title: Journal of biomedicine & biotechnology Volume: 2011 ISSN: 1110-7251 ISO Abbreviation: J. Biomed. Biotechnol. Publication Date: 2011 |
Date Detail:
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Created Date: 2011-05-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101135740 Medline TA: J Biomed Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 675701 Citation Subset: IM |
Affiliation:
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Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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