| Investigation of the potential association of vitamin D binding protein with lipoproteins. | |
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MedLine Citation:
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PMID: 20144976 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Vitamin D binding protein (DBP) acts as a vitamin D carrier and an actin scavenger. We have previously reported a correlation between serum DBP and lipid parameters in a cystic fibrosis population. In the present study, the relationship between serum DBP, lipoprotein fractions and vitamin D is investigated. METHODS: The presence of DBP in lipoprotein fractions was examined using precipitation, gel permeation chromatography and ultracentrifugation. The association between DBP and lipids was investigated in a cohort study of 211 men. Total and actin-free DBP concentrations were assessed by immunonephelometry and enzyme-linked immunosorbent assay. Serum 25(OH)- and 1.25(OH)(2)-vitamin D(3) were assayed by radioimmunoassay. Total cholesterol, high-density lipoprotein cholesterol (HDL), triglycerides and insulin were measured using routine methods. Low-density lipoprotein-cholesterol (LDL) was calculated according to Friedewald's formula. RESULTS: DBP was found to be present in very-low-density lipoprotein (VLDL). Gel permeation chromatography revealed a bimodal DBP distribution with a lipid-bound fraction besides the known free fraction. Ultracentrifugation confirmed the presence of DBP and 25(OH)-vitamin D(3) in the VLDL particle. Total serum DBP concentration and the actin-bound DBP/DBP ratio correlated significantly with total cholesterol, LDL-cholesterol, triglycerides and albumin. The 25(OH)-vitamin D(3)/DBP ratio correlated negatively with serum triglyceride concentration and body mass index (BMI). The actin-bound DBP complex was identified with Western blot. CONCLUSIONS: The lipid-bound DBP fraction may be of greater importance than initially thought. The present findings may have clinical consequences in view of the important physiological role of DBP. |
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Authors:
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Marijn M Speeckaert; Youri E Taes; Marc L De Buyzere; Armand B Christophe; Jean-Marc Kaufman; Joris R Delanghe |
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Publication Detail:
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Type: Journal Article Date: 2010-02-09 |
Journal Detail:
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Title: Annals of clinical biochemistry Volume: 47 ISSN: 1758-1001 ISO Abbreviation: Ann. Clin. Biochem. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-03-03 Completed Date: 2010-06-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0324055 Medline TA: Ann Clin Biochem Country: England |
Other Details:
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Languages: eng Pagination: 143-50 Citation Subset: IM |
Affiliation:
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Department of Clinical Chemistry, Ghent University Hospital, Gent, Belgium. joris.delanghe@ugent.be |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Aged, 80 and over Carrier Proteins / blood* Cholesterol / blood Cholesterol Esters Cholesterol, HDL / blood Cholesterol, LDL / blood Cholesterol, VLDL / blood Enzyme-Linked Immunosorbent Assay / methods Humans Lipids / blood Lipoproteins / blood* Lipoproteins, LDL / blood Lipoproteins, VLDL / blood Male Proteins / metabolism Triglycerides / blood Ultracentrifugation / methods Vitamin D / blood*, metabolism* Vitamin D-Binding Protein / blood* |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Cholesterol Esters; 0/Cholesterol, HDL; 0/Cholesterol, LDL; 0/Cholesterol, VLDL; 0/Lipids; 0/Lipoproteins; 0/Lipoproteins, LDL; 0/Lipoproteins, VLDL; 0/Proteins; 0/Triglycerides; 0/Vitamin D-Binding Protein; 0/low density lipoprotein inhibitor; 1406-16-2/Vitamin D; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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