| Investigation of natural phosphatidylcholine sources: separation and identification by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS2) of molecular species. | |
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MedLine Citation:
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PMID: 19545117 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study is a contribution to the exploration of natural phospholipid (PL) sources rich in long-chain polyunsaturated fatty acids (LC-PUFAs) with nutritional interest. Phosphatidylcholines (PCs) were purified from total lipid extracts of different food matrices, and their molecular species were separated and identified by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS(2)). Fragmentation of lithiated adducts allowed for the identification of fatty acids linked to the glycerol backbone. Soy PC was particularly rich in species containing essential fatty acids, such as (18:2-18:2)PC (34.0%), (16:0-18:2)PC (20.8%), and (18:1-18:2)PC (16.3%). PC from animal sources (ox liver and egg yolk) contained major molecular species, such as (16:0-18:2)PC, (16:0-18:1)PC, (18:0-18:2)PC, or (18:0-18:1)PC. Finally, marine source (krill oil), which was particularly rich in (16:0-20:5)PC and (16:0-22:6)PC, appeared to be an interesting potential source for food supplementation with LC-PUFA-PLs, particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). |
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Authors:
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Julie Le Grandois; Eric Marchioni; Minjie Zhao; Francesca Giuffrida; Saïd Ennahar; Françoise Bindler |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 57 ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-07-15 Completed Date: 2009-11-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 6014-20 Citation Subset: IM |
Affiliation:
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Laboratoire de Chimie Analytique et Sciences de l'Aliment, IPHC-DSA, Universite de Strasbourg, CNRS, 67400 Illkirch, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Chromatography, High Pressure Liquid / methods* Docosahexaenoic Acids / analysis Egg Yolk / chemistry Eicosapentaenoic Acid / analysis Euphausiacea / chemistry Fatty Acids, Unsaturated / analysis Food Analysis / methods* Liver / chemistry Oils / chemistry Phosphatidylcholines / chemistry*, isolation & purification Soybeans / chemistry Spectrometry, Mass, Electrospray Ionization / methods* |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Unsaturated; 0/Oils; 0/Phosphatidylcholines; 1553-41-9/Eicosapentaenoic Acid; 25167-62-8/Docosahexaenoic Acids |
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