Document Detail


Investigation of natural phosphatidylcholine sources: separation and identification by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS2) of molecular species.
MedLine Citation:
PMID:  19545117     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study is a contribution to the exploration of natural phospholipid (PL) sources rich in long-chain polyunsaturated fatty acids (LC-PUFAs) with nutritional interest. Phosphatidylcholines (PCs) were purified from total lipid extracts of different food matrices, and their molecular species were separated and identified by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS(2)). Fragmentation of lithiated adducts allowed for the identification of fatty acids linked to the glycerol backbone. Soy PC was particularly rich in species containing essential fatty acids, such as (18:2-18:2)PC (34.0%), (16:0-18:2)PC (20.8%), and (18:1-18:2)PC (16.3%). PC from animal sources (ox liver and egg yolk) contained major molecular species, such as (16:0-18:2)PC, (16:0-18:1)PC, (18:0-18:2)PC, or (18:0-18:1)PC. Finally, marine source (krill oil), which was particularly rich in (16:0-20:5)PC and (16:0-22:6)PC, appeared to be an interesting potential source for food supplementation with LC-PUFA-PLs, particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA).
Authors:
Julie Le Grandois; Eric Marchioni; Minjie Zhao; Francesca Giuffrida; Saïd Ennahar; Françoise Bindler
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  57     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-15     Completed Date:  2009-11-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6014-20     Citation Subset:  IM    
Affiliation:
Laboratoire de Chimie Analytique et Sciences de l'Aliment, IPHC-DSA, Universite de Strasbourg, CNRS, 67400 Illkirch, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Chromatography, High Pressure Liquid / methods*
Docosahexaenoic Acids / analysis
Egg Yolk / chemistry
Eicosapentaenoic Acid / analysis
Euphausiacea / chemistry
Fatty Acids, Unsaturated / analysis
Food Analysis / methods*
Liver / chemistry
Oils / chemistry
Phosphatidylcholines / chemistry*,  isolation & purification
Soybeans / chemistry
Spectrometry, Mass, Electrospray Ionization / methods*
Chemical
Reg. No./Substance:
0/Fatty Acids, Unsaturated; 0/Oils; 0/Phosphatidylcholines; 1553-41-9/Eicosapentaenoic Acid; 25167-62-8/Docosahexaenoic Acids

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