| Investigation of multidrug resistance in cultured human renal cell carcinoma cells by 31P-NMR spectroscopy and treatment survival assays. | |
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MedLine Citation:
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PMID: 15977034 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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KTCTL-26 and KTCTL-2 are renal cell carcinoma (RCC) lines with high and low expression of P-170 glycoprotein, respectively. Inherent differences between the two cell lines in terms of phosphate metabolites and growth characteristics in culture were examined for possible association with multidrug resistance (MDR). Differences in response to drug treatment were investigated for 40 h incubations with various doses of vinblastine (VBL) alone or as cotreatments with various concentrations of the calcium antagonist diltiazem (DIL) and/or interferon-alpha (IFN-alpha). Treatment effects were quantitated using the MTT survival assay and 31P magnetic resonance spectroscopy (MRS) to determine phosphate metabolite profiles in intact cells. KTCTL-2 and KTCTL-26 cells exhibited significant inherent differences in phosphocholine, glycerophosphocholine, glycerophosphoethanolamine, and phosphocreatine levels. KTCTL-26 cells were more sensitive than KTCTL-2 to 0.011 mircroM VBL alone (87% vs. 102% survival) or to 0.011 microM BL + 10 microM DIL (55% vs. 80% survival). The latter treatment resulted in a significant decrease in the ratio of phosphocholine to glycerophosphocholine in KTCTL-26 cells but no significant changes in phosphate metabolites in KTCTL-2 cells. Metabolomic 31P MRS detects different metabolite profiles for RCC cell lines with different MDR phenotypes and may be useful for noninvasive characterization of tumors in a clinical setting. |
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Authors:
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N W Lutz; S E Franks; M H Frank; S Pomer; W E Hull |
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Publication Detail:
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Type: Comparative Study; Journal Article Date: 2005-06-23 |
Journal Detail:
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Title: Magma (New York, N.Y.) Volume: 18 ISSN: 0968-5243 ISO Abbreviation: MAGMA Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-08-05 Completed Date: 2005-10-27 Revised Date: 2011-06-16 |
Medline Journal Info:
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Nlm Unique ID: 9310752 Medline TA: MAGMA Country: Germany |
Other Details:
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Languages: eng Pagination: 144-61 Citation Subset: IM |
Affiliation:
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Central Spectroscopy Department, German Cancer Research Center (DKFZ), Heidelberg, Germany. Lutz@ipb.u-strasbg.fr |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Combined Chemotherapy Protocols
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administration & dosage* Carcinoma, Renal Cell / metabolism, pathology* Cell Line, Tumor Cell Proliferation / drug effects* Cell Survival / drug effects* Diltiazem / administration & dosage Dose-Response Relationship, Drug Drug Resistance, Multiple Drug Resistance, Neoplasm Humans Interferon-alpha / administration & dosage Kidney Neoplasms / metabolism, pathology* Magnetic Resonance Spectroscopy / methods* Phosphates / analysis Phosphorus Isotopes / diagnostic use Treatment Outcome Vinblastine / administration & dosage |
| Grant Support | |
ID/Acronym/Agency:
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K08 AI050783-03/AI/NIAID NIH HHS; R01 CA113796-01A1/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Interferon-alpha; 0/Phosphates; 0/Phosphorus Isotopes; 42399-41-7/Diltiazem; 865-21-4/Vinblastine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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