Document Detail

Investigation of multidrug resistance in cultured human renal cell carcinoma cells by 31P-NMR spectroscopy and treatment survival assays.
MedLine Citation:
PMID:  15977034     Owner:  NLM     Status:  MEDLINE    
KTCTL-26 and KTCTL-2 are renal cell carcinoma (RCC) lines with high and low expression of P-170 glycoprotein, respectively. Inherent differences between the two cell lines in terms of phosphate metabolites and growth characteristics in culture were examined for possible association with multidrug resistance (MDR). Differences in response to drug treatment were investigated for 40 h incubations with various doses of vinblastine (VBL) alone or as cotreatments with various concentrations of the calcium antagonist diltiazem (DIL) and/or interferon-alpha (IFN-alpha). Treatment effects were quantitated using the MTT survival assay and 31P magnetic resonance spectroscopy (MRS) to determine phosphate metabolite profiles in intact cells. KTCTL-2 and KTCTL-26 cells exhibited significant inherent differences in phosphocholine, glycerophosphocholine, glycerophosphoethanolamine, and phosphocreatine levels. KTCTL-26 cells were more sensitive than KTCTL-2 to 0.011 mircroM VBL alone (87% vs. 102% survival) or to 0.011 microM BL + 10 microM DIL (55% vs. 80% survival). The latter treatment resulted in a significant decrease in the ratio of phosphocholine to glycerophosphocholine in KTCTL-26 cells but no significant changes in phosphate metabolites in KTCTL-2 cells. Metabolomic 31P MRS detects different metabolite profiles for RCC cell lines with different MDR phenotypes and may be useful for noninvasive characterization of tumors in a clinical setting.
N W Lutz; S E Franks; M H Frank; S Pomer; W E Hull
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2005-06-23
Journal Detail:
Title:  Magma (New York, N.Y.)     Volume:  18     ISSN:  0968-5243     ISO Abbreviation:  MAGMA     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-08-05     Completed Date:  2005-10-27     Revised Date:  2014-09-12    
Medline Journal Info:
Nlm Unique ID:  9310752     Medline TA:  MAGMA     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  144-61     Citation Subset:  IM    
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MeSH Terms
Antineoplastic Combined Chemotherapy Protocols / administration & dosage*
Carcinoma, Renal Cell / metabolism,  pathology*
Cell Line, Tumor
Cell Proliferation / drug effects*
Cell Survival / drug effects*
Diltiazem / administration & dosage
Dose-Response Relationship, Drug
Drug Resistance, Multiple
Drug Resistance, Neoplasm
Interferon-alpha / administration & dosage
Kidney Neoplasms / metabolism,  pathology*
Magnetic Resonance Spectroscopy / methods*
Phosphates / analysis
Phosphorus Isotopes / diagnostic use
Treatment Outcome
Vinblastine / administration & dosage
Grant Support
K08 AI050783/AI/NIAID NIH HHS; K08 AI050783-03/AI/NIAID NIH HHS; R01 CA113796/CA/NCI NIH HHS; R01 CA113796-01A1/CA/NCI NIH HHS
Reg. No./Substance:
0/Interferon-alpha; 0/Phosphates; 0/Phosphorus Isotopes; 5V9KLZ54CY/Vinblastine; EE92BBP03H/Diltiazem

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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