Document Detail


Investigation of metabolite alteration in dimethylnitrosamine-induced liver fibrosis by GC-MS.
MedLine Citation:
PMID:  23256471     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Background: A metabolomic study of biomarkers associated with dimethylnitrosamine (DMN)-induced hepatic fibrosis in Sprague-Dawley rats was performed using GC-MS. The clinical chemistry of the collected blood and the histopathology of excised liver samples were examined, and urine samples were prepared by solvent extraction. Results: Through pattern analysis, the DMN-treated group was divided into two subgroups based on the aspartate aminotransferase (AST) levels compared with the control, a moderately higher group (DMN subgroup A) and a significantly higher group (DMN subgroup B). Uric acid, orotic acid, N-phenylacetylglycine and glutaric acid were biomarkers for DMN subgroup A, aminomalonic acid was a biomarker for DMN subgroup B, and arabitol level distinguished control versus DMN treatment regardless of AST level. Conclusion: This study suggests that the identification and profiling of AST level-related metabolites may be useful as a diagnostic tool and for the study of the mechanism of liver fibrosis induced by DMN.
Authors:
Hyun Kyoung Ju; Ha Wook Chung; Hee-Seung Lee; Johan Lim; Jeong Hill Park; Sung Cil Lim; Joon Mee Kim; Soon-Sun Hong; Sung Won Kwon
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioanalysis     Volume:  5     ISSN:  1757-6199     ISO Abbreviation:  Bioanalysis     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101512484     Medline TA:  Bioanalysis     Country:  England    
Other Details:
Languages:  eng     Pagination:  41-51     Citation Subset:  IM    
Affiliation:
College of Pharmacy & Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Korea.
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