Document Detail


Investigation of the metabolic fate of 2-, 3- and 4-bromobenzoic acids in bile-duct-cannulated rats by inductively coupled plasma mass spectrometry and high-performance liquid chromatography/inductively coupled plasma mass spectrometry/electrospray mass spectrometry.
MedLine Citation:
PMID:  15666316     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Inductively coupled plasma mass spectrometry (ICPMS) has been used to determine the rate and routes of excretion of bromine following the intraperitoneal administration (50 mg kg(-1)) of 2-, 3- and 4-bromobenzoic acids to male bile-duct-cannulated rats. Analysis of urine and bile for (79/81)Br using ICPMS showed that all three bromobenzoic acids were rapidly excreted (82-98%) within 48 h of dosing, primarily via the urine. High-performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC/ICPMS) was then used to obtain metabolite profiles for bile and urine. These profiles revealed that extensive metabolism had taken place, with the unchanged bromobenzoic acids forming a minor part of the total of compound-related material detected. Concomitant MS studies, supplemented by alkaline hydrolysis, enabled the identification of the major metabolite of all three of the bromobenzoic acids as a glycine conjugate. Ester glucuronide conjugates were also identified, but formed only a small proportion of total.
Authors:
Berit Packert Jensen; Christopher J Smith; Christopher J Bailey; Colin Rodgers; Ian D Wilson; Jeremy K Nicholson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  19     ISSN:  0951-4198     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  2005  
Date Detail:
Created Date:  2005-02-10     Completed Date:  2005-03-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  519-24     Citation Subset:  IM    
Copyright Information:
Copyright 2005 John Wiley & Sons, Ltd.
Affiliation:
Department of Analytical Chemistry, The Danish University of Pharmaceutical Sciences, Universitetsparken 2, DK-2100 Copenhagen Ø, Denmark. bpj@dfuni.dk
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MeSH Terms
Descriptor/Qualifier:
Animals
Bile / chemistry,  metabolism
Bile Ducts / surgery
Bromobenzoates / administration & dosage,  pharmacokinetics*
Catheterization
Chromatography, High Pressure Liquid
Injections, Intraperitoneal
Male
Rats
Rats, Wistar
Spectrometry, Mass, Electrospray Ionization / methods*
Urinalysis
Chemical
Reg. No./Substance:
0/Bromobenzoates

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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