| Investigation of the metabolic fate of 2-, 3- and 4-bromobenzoic acids in bile-duct-cannulated rats by inductively coupled plasma mass spectrometry and high-performance liquid chromatography/inductively coupled plasma mass spectrometry/electrospray mass spectrometry. | |
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MedLine Citation:
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PMID: 15666316 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Inductively coupled plasma mass spectrometry (ICPMS) has been used to determine the rate and routes of excretion of bromine following the intraperitoneal administration (50 mg kg(-1)) of 2-, 3- and 4-bromobenzoic acids to male bile-duct-cannulated rats. Analysis of urine and bile for (79/81)Br using ICPMS showed that all three bromobenzoic acids were rapidly excreted (82-98%) within 48 h of dosing, primarily via the urine. High-performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC/ICPMS) was then used to obtain metabolite profiles for bile and urine. These profiles revealed that extensive metabolism had taken place, with the unchanged bromobenzoic acids forming a minor part of the total of compound-related material detected. Concomitant MS studies, supplemented by alkaline hydrolysis, enabled the identification of the major metabolite of all three of the bromobenzoic acids as a glycine conjugate. Ester glucuronide conjugates were also identified, but formed only a small proportion of total. |
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Authors:
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Berit Packert Jensen; Christopher J Smith; Christopher J Bailey; Colin Rodgers; Ian D Wilson; Jeremy K Nicholson |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Rapid communications in mass spectrometry : RCM Volume: 19 ISSN: 0951-4198 ISO Abbreviation: Rapid Commun. Mass Spectrom. Publication Date: 2005 |
Date Detail:
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Created Date: 2005-02-10 Completed Date: 2005-03-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8802365 Medline TA: Rapid Commun Mass Spectrom Country: England |
Other Details:
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Languages: eng Pagination: 519-24 Citation Subset: IM |
Copyright Information:
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Copyright 2005 John Wiley & Sons, Ltd. |
Affiliation:
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Department of Analytical Chemistry, The Danish University of Pharmaceutical Sciences, Universitetsparken 2, DK-2100 Copenhagen Ø, Denmark. bpj@dfuni.dk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bile / chemistry, metabolism Bile Ducts / surgery Bromobenzoates / administration & dosage, pharmacokinetics* Catheterization Chromatography, High Pressure Liquid Injections, Intraperitoneal Male Rats Rats, Wistar Spectrometry, Mass, Electrospray Ionization / methods* Urinalysis |
| Chemical | |
Reg. No./Substance:
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0/Bromobenzoates |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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