| Investigation into A antigen expression on O2 heterozygous group O-labeled red blood cell units. | |
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MedLine Citation:
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PMID: 18482182 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: There are two principal types of group O alleles; deletional alleles feature 261delG leading to nonfunctional truncated protein. Nondeletional alleles have the consensus guanosine at residue 261. The major nondeletional allele, O2, encodes full-length protein with Gly268Arg. While reports vary, O2 has been proposed to encode weakly functional A-glycosyltransferase (GTA). The main objective of this study was to evaluate if GTA activity is detectable in O2 donors. STUDY DESIGN AND METHODS: Donor samples from Pittsburgh and Lund were ABO typed by automated methods. DNA was extracted from 779 group O donors whose red blood cells (RBCs) were available for transfusion. ABO genotyping identified those with O2 alleles. The following tests were performed on randomly selected O2 samples (number): adsorption-elution with anti-A (3), flow cytometry (15), plasma enzyme activity (4), and attempts to convert group O RBCs to A (2) with O2 plasma and titration of plasma anti-A/-A1 (3). RESULTS: Forty O2-heterozygous donors were identified (5.1%). Adsorption-elution and sensitive flow cytometry did not reveal A antigens on O2 RBCs. Plasma enzyme analysis failed to show GTA activity above baseline; O2 plasma was unable to add measurable A antigens to O RBCs. Titers of anti-A/-A1 appeared reduced in O2 plasma but did not cause ABO typing discrepancies. No immediate hemolysis or adverse reactions were reported following transfusion of O2 RBCs to six evaluable group O recipients. CONCLUSIONS: Other than lower plasma anti-A titers, GTA activity was not found in these O2 samples. Neither automated blood grouping discrepancies nor clinical problems related to transfusing these O2 units were observed. |
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Authors:
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Mark H Yazer; Annika K Hult; Asa Hellberg; Bahram Hosseini-Maaf; Monica M Palcic; Martin L Olsson |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-05-13 |
Journal Detail:
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Title: Transfusion Volume: 48 ISSN: 0041-1132 ISO Abbreviation: Transfusion Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-08-20 Completed Date: 2008-09-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0417360 Medline TA: Transfusion Country: United States |
Other Details:
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Languages: eng Pagination: 1650-7 Citation Subset: IM |
Affiliation:
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Institute for Transfusion Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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ABO Blood-Group System
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genetics* Adsorption Alleles Blood Donors Blood Transfusion* Erythrocytes / physiology* Flow Cytometry Genotype Glycosyltransferases / metabolism Guanosine / genetics Heterozygote Humans Phenotype |
| Chemical | |
Reg. No./Substance:
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0/ABO Blood-Group System; 118-00-3/Guanosine; EC 2.4.-/Glycosyltransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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