Document Detail


Investigation of the interactions between silver nanoparticles and Hela cells by scanning electrochemical microscopy.
MedLine Citation:
PMID:  18709198     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The interactions between Hela cells and silver nanoparticles (AgNPs) have been studied by scanning electrochemical microscopy (SECM) with both IrCl(6)(2-/3-) and Fe(CN)(6)(3-/4-) as the dual mediators. IrCl(6)(2-), which can be produced in situ and react with AgNPs, is used as the mediator between the AgNPs on the cells and the SECM tip. Another redox couple, Fe(CN)(6)(3-/4-), which has a similar hydrophilicity to IrCl(6)(2-/3-), but cannot react with AgNPs, is also employed for the contrast experiments. The cell array is cultured successfully onto a Petri dish by microcontact printing (muCP) technique, which can provide a basic platform for studying of single cells. The approach curve and line scan are the two methods of SECM employed here to study the Hela cells. The former can provide the information about the interaction between Hela cells and AgNPs whereas the later gives the cell imaging. The permeability of cell membranes and morphology are two main factors which have effects on the feedback mode signals when K(3)Fe(CN)(6) is used as the mediator. The permeability of the cell membranes can be ignored after interaction with high concentration of AgNP solution and the height of the Hela cells is slightly decreased in this process. The kinetic rate constants (k(0)) between IrCl(6)(2-) and Ag on the Hela cell can be evaluated using K(3)IrCl(6) as the mediator, and they are increased with the higher concentrations of the AgNP solutions. The k(0) is changed about 10 times from 0.43 +/- 0.04 x 10(-4) to 1.25 +/- 0.07 x 10(-4) and to 3.93 +/- 1.9 x 10(-4) cm s(-1) corresponding to 0, 1 and 5 mM of AgNO(3) solution. The experimental results demonstrate that the AgNPs can be adsorbed on the cell surface and detected by SECM. Thus, the amount of AgNPs adsorbed on cell membranes and the permeability or morphology changes can be investigated simultaneously using this approach. The dual mediator system and cell array fabricated by muCP technique can provide better reproducibility because they can simplify experiments, and provide a platform for further single cell detection.
Authors:
Zhong Chen; Shubao Xie; Li Shen; Yu Du; Shali He; Qing Li; Zhongwei Liang; Xin Meng; Bo Li; Xiaodong Xu; Hongwei Ma; Yanyi Huang; Yuanhua Shao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-29
Journal Detail:
Title:  The Analyst     Volume:  133     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-08-18     Completed Date:  2008-12-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  England    
Other Details:
Languages:  eng     Pagination:  1221-8     Citation Subset:  IM    
Affiliation:
Beijing National Laboratory for Molecular Sciences, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
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MeSH Terms
Descriptor/Qualifier:
Electrochemistry / methods
Hela Cells / metabolism*,  ultrastructure
Humans
Metal Nanoparticles / chemistry*
Microchemistry / methods
Microscopy, Electron, Scanning
Silver / metabolism*
Chemical
Reg. No./Substance:
7440-22-4/Silver

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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