| Investigation of the extraction behavior of the main monoglutamate folates from spinach by liquid chromatography-electrospray ionization tandem mass spectrometry. | |
| | |
MedLine Citation:
|
PMID: 16007982 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
An LC-MS/MS method has been developed for the determination of main monoglutamate folates in spinach with folic acid as an internal standard. A sample preparation with ultrafiltration (molecular weight cut-off membrane, 5 kDa) was followed by a chromatographic run of 14.2 min, rendering the method very simple and fast. The LODs in diluted spinach matrix were 0.02, 0.09, 0.05 and 0.03 ng/mL (0.037, 0.17, 0.092 and 0.055 microg/100 g calculated according to the fresh weight of spinach) for 5-methyltetrahydrofolate, tetrahydrofolate, 5-formyltetrahydrofolate, and 10-formylfolic acid, respectively. Using this method, the extraction behaviour of the main naturally occurring monoglutamate folates has been investigated in detail. It is found that 10 min of heating at 100 degrees C, incubation with rat serum at 37 degrees C (0.05 M phosphate buffer, pH = 6.5) for 4 h and the ratio of 10 (volume of extraction buffer versus the weight of sample, mL/g) are the optimal parameters for folate extraction from spinach. The final quantitative result of the individual folates in spinach is highly influenced by the pH (from 2.9 to 8.6) of the extraction buffer. |
| | |
Authors:
|
Guo-Fang Zhang; Sergei Storozhenko; Dominique Van Der Straeten; Willy E Lambert |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Validation Studies |
Journal Detail:
|
Title: Journal of chromatography. A Volume: 1078 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2005 Jun |
Date Detail:
|
Created Date: 2005-07-12 Completed Date: 2005-09-08 Revised Date: 2009-01-15 |
Medline Journal Info:
|
Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 59-66 Citation Subset: IM |
Affiliation:
|
Laboratory of Toxicology, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antioxidants Chemical Fractionation / methods Chromatography, High Pressure Liquid / methods* Drug Stability Folic Acid / isolation & purification* Hot Temperature Hydrogen-Ion Concentration Spectrometry, Mass, Electrospray Ionization / methods* Spinacia oleracea / chemistry* Time Factors gamma-Glutamyl Hydrolase / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Antioxidants; 59-30-3/Folic Acid; EC 3.4.19.9/gamma-Glutamyl Hydrolase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A new strategy for the selective determination of D-amino acids: enzymatic and chemical modification...
Next Document: Characterization of the in vitro atropisomeric interconversion rates of an endothelin A antagonist b...