| Investigation of extracellular amino acid release in children with severe head injury using microdialysis. A pilot study. | |
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MedLine Citation:
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PMID: 12168351 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Microdialysis has been used to investigate the pathophysiology of severe head injury in adults. No similar data exist, however, for children. As part of routine neuromonitoring, five children with severe, closed head injury (GCS < 8) were fitted with a microdialysis probe (CMA 70) at the time of intracranial pressure bolt insertion. Samples were collected at hourly intervals prior to analysis of amino acids by High Performance Liquid Chromatography. In contrast to adult patients, glutamate and aspartate did not correlate with structural amino acid levels and were inversely related to glutamine levels, suggesting that excitatory aminoacid release in children with severe head injury is likely to be an active process rather than an effect of indiscriminate cell destruction and that glutamate levels extracellularly may also depend on glutamate uptake and metabolism. The nitric oxide metabolite citrulline remained at similar levels in different patients throughout the monitoring period, indicating that nitric oxide metabolism may not follow the pattern of glutamate release. Our study is suggesting that amino acid release in severe head injury in children may not correspond to the recognised patterns from cell culture and animal studies and may also differ from that in adults. |
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Authors:
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C Tolias; D Richards; N Bowery; S Sgouros |
Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Acta neurochirurgica. Supplement Volume: 81 ISSN: 0065-1419 ISO Abbreviation: Acta Neurochir. Suppl. Publication Date: 2002 |
Date Detail:
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Created Date: 2002-08-09 Completed Date: 2003-01-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100962752 Medline TA: Acta Neurochir Suppl Country: Austria |
Other Details:
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Languages: eng Pagination: 377-9 Citation Subset: IM |
Affiliation:
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Department of Neurosurgery and Institute of Child Health, Birmingham Children's Hospital, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Amino Acids / metabolism* Biological Markers / analysis Child Chromatography, High Pressure Liquid / methods Extracellular Space / metabolism* Head Injuries, Closed / metabolism, physiopathology* Humans Intracranial Pressure* Microdialysis / methods Monitoring, Physiologic / methods Pilot Projects Regression Analysis Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Biological Markers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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