Document Detail

Investigation of the environment and of mothers in transmission of rotavirus infections in the neonatal nursery.
MedLine Citation:
PMID:  18428133     Owner:  NLM     Status:  MEDLINE    
A distinct feature of neonatal rotavirus infection is the association of unusual strains that appear to be prevalent only in neonatal units and persist for long periods of time. The main aims of this study were to determine if rotavirus can be detected on environmental surfaces in the neonatal nursery and whether the infection occurs in mothers of infected and uninfected neonates. Thirty rotavirus positive neonates and an equal number of negative neonates were enrolled in this study. Stool samples from 15 mothers in each group and environmental swabs collected from the bed and surfaces around neonates were tested for rotavirus using single round and nested PCR for the VP6 gene. Rotavirus could be detected in environmental swabs using single round PCR for VP6 gene in 40% of neonates positive for rotavirus antigen by enzyme immunoassay (EIA) and 33.3% of EIA negative neonates. The detection rate was almost 100% using the nested VP6 PCR. Rotavirus was detected in maternal samples only if the nested VP6 PCR was used, with no significant difference between rates of rotavirus detection in maternal fecal samples of infected and uninfected neonates (p-0.4). Sequence analysis of nested VP6 amplicons from two environmental swabs revealed them to be closest in identity to G10P[11], the most common genotype causing infections in neonates in this setting. Interestingly, sequences of amplicons from maternal stool samples did not cluster with G10P[11] or other VP6 subgroup I strains but showed clustering with human strains of VP6 subgroup II.
Sasirekha Ramani; Rajesh Arumugam; Nithya Gopalarathinam; Ipsita Mohanty; Sudhin Mathew; Beryl Primrose Gladstone; Atanu Kumar Jana; Kurien Anil Kuruvilla; Gagandeep Kang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medical virology     Volume:  80     ISSN:  0146-6615     ISO Abbreviation:  J. Med. Virol.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-04-28     Completed Date:  2008-06-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705876     Medline TA:  J Med Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1099-105     Citation Subset:  IM    
Department of Gastrointestinal Sciences, Christian Medical College, Vellore, India.
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MeSH Terms
Antigens, Viral / analysis,  genetics
Beds / virology
Capsid Proteins / genetics
Case-Control Studies
Cross Infection / transmission*,  virology
Feces / virology
Genes, Viral / genetics
Infant, Newborn
Infant, Newborn, Diseases / virology*
Nurseries, Hospital*
Polymerase Chain Reaction
Rotavirus / classification,  genetics,  isolation & purification
Rotavirus Infections / transmission*,  virology
Sensitivity and Specificity
Reg. No./Substance:
0/Antigens, Viral; 0/Capsid Proteins; 0/VP6 protein, Rotavirus

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