| Investigation of an enantioselective non-aqueous capillary electrochromatography system applied to the separation of chiral acids. | |
| | |
MedLine Citation:
|
PMID: 10839154 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A weak anion-exchange type chiral stationary phase (CSP) based on tert.-butylcarbamoylquinine as chiral selector and silica as chromatographic support was applied to non-aqueous capillary electrochromatography. The mobile phases used consisted of acetonitrile and methanol as organic solvents, and acetic acid and triethylamine were added as background electrolytes. The influence of several experimental parameters (electrolyte concentration, acetic acid-triethylamine ratio, acetonitrile-methanol ratio and temperature) was evaluated in order to obtain improved enantioselectivity and efficiency as well as short run times for the enantiomeric separation of negatively charged chiral analytes including benzyloxycarbonyl, N-(3,5-dinitrobenzyloxycarbonyl, 9-fluorenylmethoxycarbonyl, benzoyl, acetyl and N-(2,4-dinitrophenyl) derivatized amino acids and profens. Solvent composition of acetonitrile-methanol (80:20) and enhanced electrolyte concentrations up to 600 mM acetic acid at a constant acid-base ratio of 100:1 with high applied voltages of -25 kV proved to be optimum regarding short retention times and improved efficiencies. For example, the enantiomers of Fmoc-Leu could be separated in less than 10 min with a resolution factor of 6.9 and about 100000 theoretical plates per meter. |
| | |
Authors:
|
E Tobler; M Lämmerhofer; W Lindner |
Related Documents
:
|
17417074 - Determination of fenofibric acid concentrations by hplc after anion exchange solid-phas... 15866444 - A process for the separation of 177lu from neutron irradiated 176yb targets. 20803494 - A simple chromatographic route for the isolation of meso diaminopimelic acid. 15053654 - An hplc-ms approach for analysis of very long chain fatty acids and other apolar compou... 9106494 - Mechanisms and kinetics of alpha-linolenic acid uptake in caco-2 clone tc7. 16133764 - Polyamines are absorbed through a y+ amino acid carrier in rat intestinal epithelial ce... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of chromatography. A Volume: 875 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2000 Apr |
Date Detail:
|
Created Date: 2000-09-07 Completed Date: 2000-09-07 Revised Date: 2009-01-15 |
Medline Journal Info:
|
Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 341-52 Citation Subset: IM |
Affiliation:
|
Institute of Analytical Chemistry, University of Vienna, Austria. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acids
/
chemistry,
isolation & purification* Chromatography, Micellar Electrokinetic Capillary / instrumentation, methods* Electrolytes Hydrogen-Ion Concentration Solvents Stereoisomerism Temperature |
| Chemical | |
Reg. No./Substance:
|
0/Acids; 0/Electrolytes; 0/Solvents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Separation of enantiomers by packed capillary electrochromatography on a cellulose-based stationary ...
Next Document: Non-aqueous capillary electrophoretic enantioseparation of N-derivatized amino acids using cinchona ...