Document Detail

Investigation of enantiomer recognition of molecularly imprinted polymeric monoliths in pressurized capillary electrochromatography screening the amino acids and their derivatives.
MedLine Citation:
PMID:  19486984     Owner:  NLM     Status:  MEDLINE    
Molecularly imprinted monolithic columns were prepared for chiral separation of tyrosine and its amino acid derivatives by in situ therm-initiated copolymerization of methacrylic acid, 4-vinylpyridine and ethylene glycol dimethacrylate. The enantiomers were rapidly separated on monolithic columns in less than 10 min by pressurized capillary electrochromatography (pCEC). The influences of several parameters such as the content of cross-linking monomer on the composition of the pre-polymerization mixture were systematically investigated. The influence of the pCEC conditions including the composition of the mobile phase was also optimized to obtain the good enantioseparation. It was found that in addition to molecularly imprinted recognition, chromatographic retention and electrophoretic migration play important roles in the retention and chiral recognition of molecularly imprinted polymer (MIP) columns. The cross-selectivity for similar amino acids and its derivatives were systematical investigated for understanding the recognition mechanism on the MIP monolithic columns. The results indicated that molecularly imprinted polymer recognizes the template molecule by its molecular shape defined binding cavity.
Min Li; Xucong Lin; Zenghong Xie
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-15
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1216     ISSN:  1873-3778     ISO Abbreviation:  J Chromatogr A     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-10     Completed Date:  2009-08-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  5320-6     Citation Subset:  IM    
Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
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MeSH Terms
Amino Acids / chemistry*
Capillary Electrochromatography / methods*
Tyrosine / chemistry
Reg. No./Substance:
0/Amino Acids; 55520-40-6/Tyrosine

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