| Investigation of differentially expressed proteins due to the inhibitory effects of berberine in human liver cancer cell line HepG2. | |
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MedLine Citation:
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PMID: 16880943 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The investigation of differentially expressed proteins was used together with other techniques to study the inhibitory effects of two different doses of berberine in human liver cancer cell line HepG2. For HepG2 cells treated with 24.0 mg l(-1) of berberine, an increase in the sub G(0) phase that was indicative of cell death was observed in cell cycle analysis with flow cytometry. However, no significant increase in sub G(0) was observed in HepG2 cells treated with 4.0 mg l(-1) of berberine. Using flow cytometric analysis, significant activation of caspase 3 was not observed with HepG2 cells treated with 4.0 and 24.0 mg l(-1) of berberine. In this work, labeling of cells with stable isotope was not used in the proposed method developed. The whole cell lysates from the control and treated cells were digested with trypsin and the peptides were separated by two-dimensional (cation exchange and reversed phase) liquid chromatography and tandem mass spectrometry. Our preliminary data showed that the proposed platform provided a rapid approach to study the molecular mechanism due to the inhibitory effects of different doses of the berberine on HepG2 cell lines. This included a network of proteins involved in mitogen-activated protein kinase (MAPK) phosphorelay systems, metabolism, regulation of cell cycle and DNA damage response. The differentially expressed proteins identified using the current approach were consistent with the data obtained from cell cycle analysis with flow cytometry. |
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Authors:
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Yan Lin Tan; David Goh; Eng Shi Ong |
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Publication Detail:
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Type: Journal Article Date: 2006-03-30 |
Journal Detail:
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Title: Molecular bioSystems Volume: 2 ISSN: 1742-206X ISO Abbreviation: Mol Biosyst Publication Date: 2006 May |
Date Detail:
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Created Date: 2006-08-01 Completed Date: 2006-10-25 Revised Date: 2007-06-12 |
Medline Journal Info:
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Nlm Unique ID: 101251620 Medline TA: Mol Biosyst Country: England |
Other Details:
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Languages: eng Pagination: 250-8 Citation Subset: IM |
Affiliation:
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Applied Science School, Temasek Polytechnic, Singapore, Republic of Singapore. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Berberine
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pharmacology*,
therapeutic use Cell Cycle / drug effects Chromatography, High Pressure Liquid / methods Humans Inhibitory Concentration 50 Liver Neoplasms / drug therapy Mass Spectrometry / methods Proteome / drug effects* Proteomics / methods Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Proteome; 2086-83-1/Berberine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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