| Investigation of chromatography and polymer/salt aqueous two-phase processes for downstream processing development of recombinant phenylalanine dehydrogenase. | |
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MedLine Citation:
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PMID: 19495799 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This work presents a comprehensive study between the polymer/salt aqueous two-phase systems (ATPS) and chromatography process for downstream processing of recombinant Bacillus badius phenylalanine dehydrogenase (PheDH). First, the partitioning behavior of recombinant PheDH in polyethylene glycol (PEG)/K2HPO4 ATPS was examined. For comparative purpose, a classical chromatographic protocol was performed as well. Investigation of chromatography and ATPS procedures revealed that the ATPS comprising of 9% (w/w) PEG-6000, 16% (w/w) K2HPO4 and 16% (w/w) KCl with pH of 8.0, volume ratio (V ( R )) of 0.25, temperature of 25 degrees C and 40% (w/w) cell lysate ensured the most favorable approach for PheDH downstream process. A specific activity of 4,231.4 U/mg, a yield of 96.7% and a recovery of 162.0% were obtained. Furthermore, the shorter process time (4 vs. 48 h) and the lower total cost (4 vs. 20 euro) were additionally features that confirmed the suitability of proposed technique. |
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Authors:
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Eskandar Omidinia; Hamid Shahbaz Mohamadi; Rassoul Dinarvand; Heshmat-Allah Taherkhani |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-04 |
Journal Detail:
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Title: Bioprocess and biosystems engineering Volume: 33 ISSN: 1615-7605 ISO Abbreviation: Bioprocess Biosyst Eng Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-10 Completed Date: 2010-06-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101088505 Medline TA: Bioprocess Biosyst Eng Country: Germany |
Other Details:
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Languages: eng Pagination: 317-29 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Pasteur Institute of Iran, 13164, Tehran, Iran. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Oxidoreductases
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chemistry* Biotechnology / methods* Chromatography / methods* Dose-Response Relationship, Drug Escherichia coli / metabolism Hydrogen-Ion Concentration Kinetics Molecular Weight Polyethylene Glycols / chemistry Polymers / chemistry* Recombinant Proteins / chemistry* Salts / chemistry Water / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Polyethylene Glycols; 0/Polymers; 0/Recombinant Proteins; 0/Salts; 7732-18-5/Water; EC 1.4.-/Amino Acid Oxidoreductases; EC 1.4.3.-/phenylalanine oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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