| Investigation of chicken intestinal bacterial communities by 16S rRNA targeted fluorescence in situ hybridization. | |
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MedLine Citation:
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PMID: 18568414 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of the investigation was to quantify selected dominant bacterial groups in the chicken intestinal tract. Conventional production was used as model and the effect of the supplement with Salinomycin was evaluated. Hybridization conditions were optimized for published probes with respect to a panel of reference bacteria. In chicken intestinal samples bacteria were quantified by fluorescence in situ hybridization with 16S rRNA oligonucleotides directed towards bacteria related to Lactobacillus, Bacillus, Enterococcus-Streptococcus-Lactococcus, Enterobacteriaceae, Bacteroides, Clostridium and the domain Bacteria in lumen of ileum and cecum as well as on the intestinal wall including mucus of four individuals. Salinomycin in feed reduced counts of the Lactobacillus-, Enterobacteriaceae- and Clostridium-like bacteria in lumen of ileum compared to the conventional control. Increased or decreased bacterial counts were registered by Salinomycin in the ceca compared to the control. Relatively higher counts of Bacteroides- and Clostridium-like bacteria were found on the intestinal wall including mucus compared to lumen. The increase in numbers of some bacterial groups as well as the expected reduction by Salinomycin and the observed difference in the relative distribution of bacteria between lumen and intestinal wall are new observations that will need further investigation. |
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Authors:
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K N Olsen; M Henriksen; M Bisgaard; O L Nielsen; H Christensen |
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Publication Detail:
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Type: Journal Article Date: 2008-06-21 |
Journal Detail:
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Title: Antonie van Leeuwenhoek Volume: 94 ISSN: 0003-6072 ISO Abbreviation: Antonie Van Leeuwenhoek Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-08-26 Completed Date: 2008-12-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372625 Medline TA: Antonie Van Leeuwenhoek Country: Netherlands |
Other Details:
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Languages: eng Pagination: 423-37 Citation Subset: IM |
Affiliation:
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Department of Veterinary Pathobiology, Faculty of Life Sciences, University of Copenhagen, Stigbøjlen 4, Frederiksberg C, Denmark. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animal Feed Animals Bacteria / classification, drug effects, genetics*, isolation & purification* Base Sequence Chickens / microbiology* Ecosystem Female In Situ Hybridization, Fluorescence Intestines / microbiology Male Oligonucleotide Probes / genetics Pyrans / administration & dosage RNA, Bacterial / genetics* RNA, Ribosomal, 16S / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Oligonucleotide Probes; 0/Pyrans; 0/RNA, Bacterial; 0/RNA, Ribosomal, 16S; 53003-10-4/salinomycin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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