Document Detail

Investigation on cell proliferation with a new antibody against thymidine kinase 1.
MedLine Citation:
PMID:  11790855     Owner:  NLM     Status:  MEDLINE    
The cytosolic thymidine kinase 1 (TK1) is one of the enzymes involved in DNA replication. Based on biochemical studies, TK1 is activated at late G1 of cell cycle, and its activity correlates with the cell proliferation. We have developed a polyclonal anti-TK1 antibody against a synthetic peptide from the C-terminus of human TK1. Using this antibody, here we demonstrate the exclusive location of TK1 in the cytoplasm of cells. Cell cycle dependent TK1 expression was studied by simultaneous fluorescence staining for TK1 and bromodeoxyuridine, by using elutriated cells, and by quantitation of the amount TK1 in relation to the cellular DNA content. TK1, which was strongly expressed in the cells in S+G2 period, raised at late G1 and decreased during mitosis. The amount of TK1 increased three folds from late G1 to G2. TK1 positive cells were demonstrated in areas of proliferation activity of various normal and malignant tissues. The new anti-TK1 antibody works in archival specimens and is a specific marker of cell proliferation.
N Wang; Q He; S Skog; S Eriksson; B Tribukait
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology     Volume:  23     ISSN:  0921-8912     ISO Abbreviation:  Anal Cell Pathol     Publication Date:  2001  
Date Detail:
Created Date:  2002-01-15     Completed Date:  2002-02-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8911016     Medline TA:  Anal Cell Pathol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  11-9     Citation Subset:  IM    
Department of Oncology and Pathology, Karolinska Institute, Stockholm, Sweden.
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MeSH Terms
Antibodies / chemistry*
Antimetabolites, Antineoplastic / pharmacology
Bromodeoxyuridine / pharmacology
Carcinoma, Ehrlich Tumor / metabolism
Cell Cycle
Cell Division
Cells, Cultured
Cytosol / enzymology
Flow Cytometry
G1 Phase
G2 Phase
Hela Cells
In Situ Hybridization
Microscopy, Fluorescence
Neoplasm Transplantation
Peptides / chemistry
Protein Structure, Tertiary
S Phase
Thymidine Kinase / immunology*,  metabolism*
Tissue Distribution
Tumor Cells, Cultured
Reg. No./Substance:
0/Antibodies; 0/Antimetabolites, Antineoplastic; 0/Peptides; 59-14-3/Bromodeoxyuridine; EC Kinase; EC kinase 1

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