Document Detail


Investigation of carbon nanofibers as support for bioactive substances.
MedLine Citation:
PMID:  18709446     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this paper we have studied the adsorption properties of various bio-active systems onto the surface of carbon nanofibers (CNF) synthesized by chemical vapor deposition (CVD). Amino acids (alanine, aspartic acid, glutamic acid) and glucose oxidase (GOx) were adsorbed on CNF and the results were compared with those obtained when activated carbon (AC) was used as support. CNF and AC properties (hydrophilic or hydrophobic properties) were characterized by the pH value, the concentration of acidic/basic sites and by naphthalene adsorption. CNF with immobilized GOx was additionally investigated as a highly sensitive glucose biosensor. An amperometric method was used in an original manner to detect the changes in the specific activity of GOx, immobilized longer time on CNF. The method demonstrates that not the whole enzyme adsorbed onto CNF can catalyze the oxidation of glucose from the solution.
Authors:
Liliana Olenic; Gheorghe Mihailescu; Stela Pruneanu; Dan Lupu; Alexandru R Biris; Petre Margineanu; Sorina Garabagiu; Alexandru S Biris
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Publication Detail:
Type:  Journal Article     Date:  2008-08-16
Journal Detail:
Title:  Journal of materials science. Materials in medicine     Volume:  20     ISSN:  0957-4530     ISO Abbreviation:  J Mater Sci Mater Med     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-16     Completed Date:  2009-04-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9013087     Medline TA:  J Mater Sci Mater Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  177-83     Citation Subset:  IM    
Affiliation:
National Institute for R&D of Isotopic and Molecular Technologies, Cluj-Napoca, Romania. olenic@itim-cj.ro
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Amino Acids / chemistry
Biocompatible Materials / chemistry*
Biosensing Techniques
Carbon / chemistry*
Coated Materials, Biocompatible / chemistry
Enzymes, Immobilized / chemistry,  metabolism
Glucose Oxidase / chemistry,  metabolism
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Nanostructures / chemistry*,  ultrastructure
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Biocompatible Materials; 0/Coated Materials, Biocompatible; 0/Enzymes, Immobilized; 7440-44-0/Carbon; EC 1.1.3.4/Glucose Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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