| Investigation of carbon nanofibers as support for bioactive substances. | |
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MedLine Citation:
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PMID: 18709446 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this paper we have studied the adsorption properties of various bio-active systems onto the surface of carbon nanofibers (CNF) synthesized by chemical vapor deposition (CVD). Amino acids (alanine, aspartic acid, glutamic acid) and glucose oxidase (GOx) were adsorbed on CNF and the results were compared with those obtained when activated carbon (AC) was used as support. CNF and AC properties (hydrophilic or hydrophobic properties) were characterized by the pH value, the concentration of acidic/basic sites and by naphthalene adsorption. CNF with immobilized GOx was additionally investigated as a highly sensitive glucose biosensor. An amperometric method was used in an original manner to detect the changes in the specific activity of GOx, immobilized longer time on CNF. The method demonstrates that not the whole enzyme adsorbed onto CNF can catalyze the oxidation of glucose from the solution. |
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Authors:
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Liliana Olenic; Gheorghe Mihailescu; Stela Pruneanu; Dan Lupu; Alexandru R Biris; Petre Margineanu; Sorina Garabagiu; Alexandru S Biris |
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Publication Detail:
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Type: Journal Article Date: 2008-08-16 |
Journal Detail:
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Title: Journal of materials science. Materials in medicine Volume: 20 ISSN: 0957-4530 ISO Abbreviation: J Mater Sci Mater Med Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2009-01-16 Completed Date: 2009-04-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9013087 Medline TA: J Mater Sci Mater Med Country: United States |
Other Details:
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Languages: eng Pagination: 177-83 Citation Subset: IM |
Affiliation:
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National Institute for R&D of Isotopic and Molecular Technologies, Cluj-Napoca, Romania. olenic@itim-cj.ro |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption Amino Acids / chemistry Biocompatible Materials / chemistry* Biosensing Techniques Carbon / chemistry* Coated Materials, Biocompatible / chemistry Enzymes, Immobilized / chemistry, metabolism Glucose Oxidase / chemistry, metabolism Microscopy, Electron, Scanning Microscopy, Electron, Transmission Nanostructures / chemistry*, ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Biocompatible Materials; 0/Coated Materials, Biocompatible; 0/Enzymes, Immobilized; 7440-44-0/Carbon; EC 1.1.3.4/Glucose Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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