Document Detail


Investigation of the abnormal low-density lipoproteins occurring in patients with obstructive jaundice.
MedLine Citation:
PMID:  183749     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The abnormal lipoproteins of the density range 1.019-1.063g/cm3 occurring in the plasma of patients with obstructive jaundice were studied. Subfractionation of this density class by combined sodium phosphotungstate precipitation, ultracentrifugation, and column chromatography on hydroxyapatite and agarose gel yielded essentially three fractions: (1) lipoprotein-X, (2) A triglyceride-rich lipoprotein for which we propose the term lipoprotein-Y and (3) an abnormal lipoprotein, lipoprotein-B. Marked differences between these fractions with respect to electron-microscopic appearance, hydrated densities, chemical composition and immunochemical characteristics were observed. The protein moiety of lipoprotein-X consisted primarily of apolipoprotein-C and albumin. Lipoprotein-Y showed, in addition to apolipoprotein-C, the presence of apolipoprotein-B. The 'lipoprotein-B' fraction isolated from sera of these patients had higher triglyceride and free cholesterol contents than that of normal individuals and an unusually high content of apolipoprotein-C. The relative distribution of lipoprotein-X, lipoprotein-Y and 'lipoprotein-B' varied from patient to patient. The importance of considering the existence of lipoprotein-Y in screening patients for cholestasis by the lipoprotein-X test is discussed.
Authors:
G M Kostner; P Laggner; H J Prexl; A Holasek
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  157     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1976 Aug 
Date Detail:
Created Date:  1976-10-29     Completed Date:  1976-10-29     Revised Date:  2010-09-01    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  401-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cholestasis / blood*
Cholesterol / analysis
Humans
Immunoelectrophoresis
Lipoproteins, LDL / blood*,  isolation & purification
Phospholipids / analysis
Proteins / analysis
Triglycerides / analysis
Chemical
Reg. No./Substance:
0/Lipoproteins, LDL; 0/Phospholipids; 0/Proteins; 0/Triglycerides; 57-88-5/Cholesterol
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